安祖花愈伤组织增殖与分化培养基的优化

1,1,2(1.,300192;2.,300072)[][],6-BANAA,,[]:MS(1/2NH4NO3+1/2CaCl2),4.976-BANAA,,6-BA0.75mg/L,,NAA0.050.2mg/L[]:MS(1/2NH4NO3+1/2CaCl2)+0.75mg/L6-BA+0.1mg/LNAA+30g/L+7.0g/L(pH5.86.0);;;6-BA;NAA;;S682.1+4A0517-6611(2008)21-08925-03OptimizingMediumforProliferationandDifferentiationofAnthuriumCallusYANGXiao-lingetal(TianjinAcademyofAgriculturalScience,Tianjin300192)Abstract[Objective]TheaimwastooptimizethemediumforproliferationanddifferentiationofAnthuriumcallus.[Method]WithAnthuriumcallusasmaterial,theeffectofbasicmedium,exogenoushormone,6-BAandNAAonthequalityofproliferationanddifferentiationanddifferentiationseedlingsofAnthuriumcalluswasstudied,thecontentchangeofendogenoushormoneswasdetected,andphenotypicfeaturesofdifferentiationseedlingswereob-served.[Result]ThesuitablebasicmediumofproliferationofAnthuriumcalluswas:theimprovedMS(1/2NH4NO3+1/2CaCl2),proliferationmultiplewasupto4.97times.Bothof6-BAandNAAimpactedtheproliferationanddifferentiationofAnthuriumcallus,whentheconcentrationof6-BAwas0.75mg/L,theproliferationofcalluswasfast,andqualityofdifferentiationseedlingswasgoodundertheappropriatetemperaturecondition.TheproliferationofAnthuriumcallusandNAAconcentrationrangeofsuitabledifferentiationwas0.05-0.2mg/L.Thechangeofendogenoushormoneswasarisenbyvari-ationofexogenoushormoneofAnthuriumcallus.[Conclusion]ThesuitablebasicmediumofproliferationanddifferentiationofAnthuriumcalluswasMS(1/2NH4NO3+1/2CaCl2)+0.75mg/L6-BA+0.1mg/LNAA+30g/Lsugar+7.0g/Lagarpowder(pH5.8-6.0).KeywordsAnthuriumcallus;Proliferation;Differentiation;6-BA;NAA;Endogenoushormone(1968-),,,,,2008-05-19(AnthuriumandraeanumLind.),,,2,[1][2-3],,,[4-6],,,11.1,MS+0.1mg/LNAA+0.5mg/L6-BA,1.21.2.16,(G1)6,:MS;:MS1(1/2NH4NO3);:MS2(1/2NH4NO3+1/2CaCl2);:MS3(1/4NH4NO3+1/2CaCl2);:KC+MS+MS+150ml/L;:KC+MS+MS+MS12,2620,16h/d,75d(G2),1.2.26-BA4,(G1)4,MS2(1/2NH4NO3+1/2CaCl2)+0.1mg/LNAA+30g/L+7.0g/L(pH5.86.0),46-BA,:0.25mg/L6-BA;:0.5mg/L6-BA;:0.75mg/L6-BA;:1mg/L6-BA36,182621,16h/d,182518,16h/d,75d(G2),,1.2.3NAA4,(G1)4,MS2(1/2NH4NO3+1/2CaCl2)+0.75mg/L6-BA+30g/L+7.0g/L(pH5.86.0),4NAA,:0.05mg/LNAA;:0.1mg/LNAA;:0.2mg/LNAA;:0.4mg/LNAA1275d,(G2)1.2.4,,4,1.2.242(75d1,6),,2,1,1MS2(1/2NH4NO3+1/2CaCl2),2,,,6,3,JournalofAnhuiAgri.Sci.2008,36(21):8925-89271.3=(G2-G1)/G1(1)=/G2(2)=/(3)22.11,,;KC,MS,2.4,KC1.6;MS,,4.97;,;MS1/4,,MS2(1/2NH4NO3+1/2CaCl2)2.26-BA2,,6-BA,6-BA6-BA0.75mg/L,7.25,6-BA0.250.5mg/L(P0.05)6.1,(P0.01),2518,6-BA(P0.05),,MS2(1/2NH4NO3+1/2CaCl2),0.75mg/L6-BA,,1Table1EffectsofbasicmediumonthecallusproliferationmultipleandseedlingdifferentiationofAnthuriumandraeanumTreatmentCallusproliferationmultipleDifferentiatedseedlingnumberpergramcallus2.4513.34.6615.44.9714.03.729.31.4615.31.587.726-BATable2Effectsof6-BAoncallusproliferation,differentiatedseedlingnumberandsingleseedlingweightunderdifferentconditionsTreatment26,21Daytemperatureofmorethan26,nighttemperatureofmorethan21ProliferationmultipleDifferentiatedseedlingnumberpergramcallusgSingleseedlingweight25,18Daytemperatureoflowerthan25,nighttemperatureoflowerthan18ProliferationmultipleDifferentiatedseedlingnumberpergramcallusgSingleseedlingweight3.21b12.9A0.128b1.785.10.0543.83b11.8A0.124b2.105.70.0447.25a6.1B0.194a2.235.20.0716.17ab4.9B0.178a2.636.20.064:0.01,0.05Note:Capitallettersmeansignificantdifferenceat0.01levelandsmalllettersmeansignificantdifferenceat0.05level.3NAATable3EffectsofNAAoncallusproliferationandseedlingdifferentiationTreatmentProliferationmultipleSituationsoftest-tubeseedlings6.0,34,,,7.7,45,,,7.45,45,,6.48,,2.3NAA3,6-BA0.75mg/L,NAA0.050.4mg/L,(P0.05),6.07.7NAA0.2mg/L,;4,,NAA0.4mg/L,,,,NAA0.050.2mg/L2.44,6-BANAA,,6-BANAA51(),,;6-BANAA7.51(),,2,MS,6-BANAA5,2MS,1,,,,62982008,(4),MS,,MS,,,186-BA0.251mg/LNAA0.1mg/L46-BANAA4Table4Effectsofexogenoushormones6-BAandNAAonthecontentsof4kindsofendogenoushormonesincalliCulturetimeTreatmentContinuoussubcultureofhormone-containingmediaAuxinCytokininGibberellinAbscisicacidMSCultureoftransferringhormone-containingmediatoMSmediawithouthormonesAuxinCytokininGibberellinAbscisicacid1686.4818.1083.3090.901-6months74.1023.1990.2586.0860.9026.8084.0666.9054.1024.6673.0574.38712130.8821.78112.9584.0066.1812.1278.7053.707-12months125.7827.78114.1483.5651.4714.0587.7051.4876.6030.1492.0357.0439.1923.0075.8550.8073.4428.8097.9572.7636.2717.2062.5052.701318126.7031.3257.9082.3013-18months112.7035.3449.2267.8790.9653.3552.3570.1074.3051.4142.7362.85:3Note:Thedatainthetablearethemeansof3repetitionsforeachtreatment.5Table5Phenotypedifferencebetweenthehormone-containingmediaandthemediawithouthormoneTreatmentCultureofhormone-containingmediaMultiplicationcoefficientofcalliSeedlingnumberpergramcallusgSingleseedlingweightMSTransferringhormone-containingmediatoMSmediawithouthormonesMultiplicationcoefficientofcalliSeedlingnumberpergramcallusgSingleseedlingweight1.226.440.0530.3822.260.0681.328.710.0430.3415.190.1142.035.210.0710.119.960.0811.255.670.063-0.03912.960.0883,1/2MS,,MS[4-6],,,MS2(1/2NH4NO3+1/2CaCl2)+0.75mg/L6-BA+0.1mg/LNAA+30g/L+7.0g/L(pH5.86.0)[1],,.[J].,2003(2):11-12.[2],