DNA甲基化和肿瘤的关系

DNADNA5-mCpGDNA60%5CpG,CpGmCpGXCpGCpGDNADNADNACT,CpGCTCpGDNATATAGGGCGAATTGGGCCCTCTAGATGCATGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTGCCCTTTAGTATTGTTTGGTGAAATGGTACGTGTTTATAATTTTAGTTATTTAGGAGGTTGAGGTAGGAGGATTTTTTGAGTTTAGGAGTTTAAGTTTAGTTTGGGTAATATAGTTTAGTGGTTATATTAAAAAAAGTAAAATAGTCGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCGGGTGGATTACGAGGTTAGGAGGTTGAGATTATTTTAAGGGCAATDNADNADNAmCpGmCpGDMAP1TSG101Mi2DNAH3H4,DNA,CpG(MeCPs)DNAmCpG,(HDAC)DNADNADNADNADNA:DNA,5-,,CTp53Rbc-H-ras-1,DNA,1.ERERER1CpGER,1/3ER,ER,ER,ERSouthernMS-PCR,ER,MCF-7T47-DZR75-1,ERMDA-MB-231MDA-MB435MDA-MB-468Hs578t,50%ER,DNAERDNA1(DNMT1),ERERDNMT1RNAERDNMT1,ERDNMT1S2.PRPR1CpGhPRahPRbN-hPRbER,hPRaSouthernMS-PCRPR,40%PRDNMT15-aza-DcPRMSA-MB-231,PR3.BRCA1BRCA11994,,,50%BRCA1,BRCA1DNABRCA1Southern(MS-SSCA)BRCA1,1124.2%BRCA1,BRCA14.(E-cadherin)E-cadherin,,E-cadherin,50%MDAMB-435,MS-PCRE-cadherin5CpG,E-cadherinE-cadherin30%,60%,E-cadherinE-cadherin5.P16INK4a/CDKN2A/MTSDNAP16INK4a4(CDKI4),Rb/G1SP16INK4aP19ARFP16INK4a,1.9%1.0%20%30%T47-DHMECsZR75-1514030%P16INK4a6.syk,syk,B(BcR),sykB,C(PLC)-23(PI3-K)sykBPLC-2sykERKJNK,,sykAktPI3-Ksyk,Tsyk-,-,RT-PCRMS-PCR4015sykmRNAsyksykmRNA,,,sykmRNAsykmRNA,sykmRNAsykOkamuraSykp53p53syk,CartersykHER2/neu/HER2/neusykHER2/neuMahabeleshwarsyk3(PI-3)NF-B(u-PA),u-PA7.Nm23-H1Nm23-H12CpGDNA5-Aza-CdR115Nm23H13Nm23-H18.hDAB2IPDOC-2/DAB2interactiveproteinhDAB2IPRasGTPasehDAB2IPhDAB2IP5-aza-2-deoxycytidinehDAB2IPm2am2bm2a251144%391538%m2b251248%391333%m2b9.14-3-3PHME1(humanmammaryepithelial1)RAR2()DNADNACyclinD2,RAR-?,Twist,RASSF1A,HIN-1(25)12810CyclinD2,RAR-?,Twist,RASSF1A,HIN-1HIN-1HIN-1RAR-?RASSF1A,APC,DAP-kinaseRASassociationdomainfamilyprotein1A(RASSF1A),adenomatouspolyposiscoli(APC),death-associatedproteinkinase(DAP-kinase)RASSF1A,APC,DAP-kinase343294%RASSF1A2265%APC1547%DAP-kinase1750%UrokinaseuPAuPA()uPAS-Adenosyl-L-methionine(AdoMet)uPA5-azacytidineAdoMetuPA.DNADNA,,.DNADNADNMTHDACDNMT5-aza-C5aza-dC,DNADNADNMTDNMT,,DNMT,HT295-aza-DcSTAT-123