基因检测相关概念

1DNADNA100020PCRDNAPCR2DNAgenemutation1DNADNA3PCRDNADNAPCRDNADNAPCR1983Mullis1985DNAPCR2013PCR1973TaqDNAPCRPCRDNAPCR----DNADNA93DNAPCRDNADNADNA55DNADNA--72DNATaqDNAdNTPDNA----24234(genechip)DNA80TATGCAATCTAG5genotypeallelegeneticpolymorphism3DNADNA6DNADNADNAmethylationDNADNADNADNADNACG55'-CG-3'DNA5'DNADNADNADNADNADNAB-DNAZ-DNAZ-DNAMBD/MeCPCpGDNA55-mCN6-N6-mA77-mG7geneamplificationRNARNADNADNArRNA400081GFPGUSLacZLuciferasese23AB4cDNA.9proteinPhosphorylationPO4ATPGTP10DNA2080Marshfield(MarshfieldMedicalResearchFoundation)James(OregonHealthSciencesUniversity)WisDNADNADNA(ShortTandemRepeats,STRs)(SimpleSequenceRepeat,SSRs),16bpDNA3(pure),(compound)(interrupted),::ATATATATATATATATATATATAT:ATATATCACACACACACACAC:ATATATCAATATATCAATATATADNA.DNA(strandslippage)DNA(polymorphicinformationcontent,PIC).(geneticmarker)MSI11circulatingtumorcellCTCCTCCirculatingTumorCellCTCCTCCTC12GenotypingGenotypeGenotypicassayPCRDNAASOprobes13DNASNPIndel(expressedregion)(Splicing)RNARNA180,0001%30MB14