XXXX年仪器设备-液质联用(LC-MS)目录

file:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]LTQOrbitrapLTQOrbitrapLTQLTQ-OrbitrapMSnTOFLTQOrbitrapLTQOrbitrapFinniganLTQLTQLTQMSMSnLTQC-(C-trap)C-trapOrbitrapOrbitrapC-trapLTQOrbitrapDataDependent“Bottom-up”LTQOrbitrapLTQOrbitrapOrbitrapLTQOrbitrap60000MSMSnMSn1.2.LC/MSn3.©2005-2009()9892006,200031,021-60896520,[e-mail]/APCICE/MSLTQOrbitrapIonTrapElectro-SprayMassSpectrometryEsquire2000,3000,HCTfile:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]1.2.3.4.LC-MS/MS1.25pg5pg2.27,000amu/sec3.upto6000m/z4.11©2005-2009()9892006,200031,021-60896520,[e-mail]●QuattroPremierLC/MS/MS●●●1.2-42.(ESCiTM,APPI/IonSabre)3.T-WaveTM4.(GLP/21CFRPart11,IQ/OQ/PQ)5.2/3©2005-2009()9892006,200031,021-60896520,[e-mail]300file:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]320-MSVarian320-MS1.APIVarian320-MSVarianSelecTempTMAPIVarian2.Varian320-MSAPILCMSX,Y320-MS3.4.MSMS/MSCID,5.10ms6000Da/Sec300ms2-chlrobiphenyl188152(25eV)200pptinjected6320-MS2000Da7v6.88320-MSLCGC97210©2005-2009()9892006,200031,021-60896520,[e-mail]:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]LTQ1.ETD)2.PQD)3.MS/MSMS34.(CNL)MetworksTMMassFrontierLTQXLIonMaxESI(APCI(APPIIonMaxESIAPCIxyzESI(APCI(APPIETD),NSI).AccelaLTQXLPQD(ETD(LTQXL/(ETD)LTQXLETDCIDPTMCIDETD.LTQCNL)MetWorksMassFrontierETDLTQXLLTQLTQXLETDETDECDPTMETD©2005-2009()9892006,200031,021-60896520,[e-mail]-freeMSnOrbitrapXLMSMSn100,000ETD/ThermoFisherScientificLTQOrbitrap®(ETD)(MALDI).MALDILTQOrbitrap,.ETDLTQOrbitrapXL.ETDLTQOrbitrapXL,,./,(PTM)(),Top-down’Middle-downMALDILTQOrbitrapXLDiscovery.MS,MS/MSMSn50,000FWHM1-2ppm.LTQOrbitrap,MALDILTQOrbitrapXL™file:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]2D,,iTRAQ™,.ThermoScientificLTQOrbitrapDiscoveryLTQOrbitrapQ-TOFMSnLTQOrbitrapDiscovery30KLTQOrbitrapXLMSMSnLTQOrbitrapLTQOrbitrapXLHCDMS/MSiTRAQTMPTMdenovoETDLTQOrbitrapHCDlabel-free©2005-2009()9892006,200031,021-60896520,[e-mail]-/(HPLC-Chip/MS)——--/3,50020C18/6000--AnalyticalChemistry;Electrophoresis,PNS*#1*#2**********-(HPLC-ChipMS)-(HPLC-ChipMS)file:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]©2005-2009()9892006,200031,021-60896520,[e-mail]500-MSEnhanceChargeCapacityECC™3D1010TripleResonanceScanning++10“”“”500-MS1——SelecTemp™SelecFlow™,2——3ECC™——4——5200——/1502000u50100000u20.5u3ESIMS/MS250fgS/N≥100:1415000u/s5MSnn=10©2005-2009()9892006,200031,021-60896520,[e-mail]100/500-MS-Uniquefile:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]LC-TOFMSESIESIAPCI100/50010-1810pg:5ppm,:1uL/min:6000amu:100:LowFlowElectrospray:Flowratesfrom0.1μL/minuteto2μL/minuteHighFlowElectrospray:Flowratesfrom10μL/minuteto1.0mL/minuteAPCI:Flowratesfrom10μL/minuteto1.5mL/minute:500(attomole,10-18mol),1μL10pg100μL/minute©2005-2009()9892006,200031,021-60896520,[e-mail]100000ExactiveTM10ExactiveTM----ExactiveTM1ExactiveTM10ExactiveLCMS-FTfile:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]ExactiveTM1ThermoScientificExactive-10ExactiveQrbitrapTMExactiveTMExactiveTM1231000004©2005-2009()9892006,200031,021-60896520,[e-mail]8200LC/TOFMSTOFMSLC3600HPLCHPLCLC/MS1oaRTOF2m10000FWHM,@1001800u2Micro/NanoSpray3100.548000u5100000.1u61001800u78L/S200L/S880×60×130cm××80cm75kgPC©2005-2009()9892006,200031,021-60896520,[e-mail]8200LCTOFMSAgilent6530-file:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]-6530QTOFMS-1.•0.1~2.5mL/min••RF•MS/MS•“”2.•QTOFQTOF••3.•(ADC):ADCTDC105MSMS/MS•6530QTOFMS4GHzADC0.25ns•6530QTOFMSADC32gigabit/sec20.5~10s6530QTOFMS20spectra/sec10MS/MSspectra/sec4.6530QTOFMS6530QTOFMSMassHunterWorkstationQTOFMSMassHunterWorkstationMS/MS•MS/MSfile:///G|/OPW/pp/download/bihec_pdf_bycat/2010-(LC-MS).htm[2010-4-2620:21:53]•/molecularfeatureextraction,MFE:••••MassDefect•••••MGF(Mascot),JCAMP,MZxml,andMZml.CompoundsummarycsvMSMS/MS–SpectrumMillMSProteomicsWorkbench–Bioconfirmationsoftware–MetaboliteIdentificationsoftware–MassProfilingsoftwareandGenespringMSsoftware:BiomarkerMS1pg(M+H)+m/z609.2807;LC/MS400µL/minLC/MS400µL/min10:1RMSMS/MS1pg(174,195,397,448m/z);LC/MS400µL/min50:1RMS(typical200:1RMS)MSNanoflowChipMS,300nL/min500attomoleofBSApeptidedigest(200attomole-typical)15%coverageinproteindatabasesearch(PMF)MS/MSNanoflowChipMS,300nL/min100fmol/ulGlu-fibpeptide50:1RMS20,000FWHMMS(M+H)+(m/z609.2807)20pgLC/MS400µL/min2ppmMS/MSm/z60939720pgLC/MS,400µL/min5ppmMSInfusionusingnanoflowChipMS300nL/minGlu-fibpeptide(M+2H)++(m/z785.83)2ppmfile:///G|/O