蚕蛹的营养成分分析-王彦平

3[4-5],,,,,8,-6.00D,,:MT1MT2MT3MT1MT1,MT1,(P0.05),Alarma-Estrany[2],,,,,,(P0.05),,[1]RadaJA,WiechmannAF.MelatoninReceptorsinchickoculartissues:miplicationsforaroleofmelatonininoculargrowthreg-ulation[J].InvestOphthamlolandVisSc,i2006,47(1):25[2]Alarma-EstranyP,PintorJ.Melatoninreceptorsintheeye:location,secondmessengersandroleinocularphysiology[J].PharmacolTher,2007,113(3):507[3],,.[J].,2007,29(4):36[4]WallmanJ,AdamsJI.Developmentalaspectsofexperimen-talmyopiainchicks:susceptibility,recoveryandrelationtoemmetropization[J].VisionRes,1987,27:1139[5],,.[J].,2001,3:103[6],.[J].,2008,8(8):1657(2008-08-27)王彦平1),刘洁2),吴予明2),刘利娥2)#,吕全军1),吴拥军2)1)4500012)450001#,,1966,,:,E-mai:[e-mail]关键词;;中图分类号R914摘要:,:(105),,,,,(AAS)(CS)(EAAI),:11.28%,20.77%70.69%,1459.68mg/100g,849.65%,(NEAA)0.99,(2.14mg/100g)AASCS,EAAI111.14,,:638()20095443,AnalysisofnutritioncompositiononsilkwormpupaWANGYanping1),LIUJie2),WUYuming2),LIULie2),LVQuanjun1),WUYongjun2)1)DepartmentofNutritionandFoodHygiene,CollegeofPublicHealth,ZhengzhouUniversity,Zhengzhou4500012)DepartmentofSanitaryChemistryandHealthInspection,CollegeofPublicHealth,ZhengzhouUniversity,Zhengzhou450001Keywordssilkwormpupa;nutritioncomposition;reversedphasehighperformanceliquidchromatographyAbstractAmi:Toanalyzeandevaluatethenutritioncompositionofsilkwormpupa,soastoprovideevidenceforde-velopingnewresourcefood.Methods:Thewatercontentofsilkwormpupawasdeterminedbynormalpressuredryingmeth-od(105).Theamountofcrudefatwasdeterminedbysoxhletmethod,andcrudeprotein,byKjeldahlapparatus.Thecontentsofaminoacidsweredeterminedbyreversedphasehigh-performanceliquidchromatography(RP-HPLC),themetalelementcontents,byatomicabsorptionspectrophotometry,andthephosphoruscontent,bydynamicpotentiometry.Thequa-lityofaminoacidswasevaluatedbyaminoacidsscore(AAS),chemicalscore(CS)andessentialaminoacidindex(EAAI),andthentheseresultswerecomparedwithothermostcommonanimalfoods.Results:Thecontentofwaterinsilkwormpupaafterfilaturewas11.28%.Thecrudefatandproteinoccupied20.77%and70.69%ofitsdryweightre-spectively.Thetotalcontentof14aminoacidswas59.68mg/100g.Eightessentialaminoacids(EAA)accountedfor49.65%oftotalaminoacidandtheratioofEAAandnon-essentialoneswas0.99.Besides,itwasrichintaurine(2.14mg/100g).AccordingtoAASandCS,thefirstandsecondlimitedaminoacidwereleucine(Leu)andmethionine(Met)respectively.EAAIwas111.14.Mineralelementswererichinsilkwormpupa,whichwasmuchhigherthanthatofothercommonaminalfoods.Conclusion:Silkwormpupaisanewresourcefoodwithrichernutritionandhasvastprospectsfordevelopingandapplicating.(silkwormpupa),BombyxmoriL.,[1],30,80%,,[2],,,,,,11.1,,,6024h,1.2:,GB5009.3-85:,GB5009.6-85:,GB5009.5-851.36mol/LHCl(0.1%[3]),10024h,OPA[4],,Agilent1100(Ag-ilent);Hyper-silODS-BPC18;1.4(HNO3HClO441),1%Z-5000(HITACHI);,()[5],1%0.1%TritonX-100(11);PVCMG[6]()639JournalofZhengzhouUniversity(MedicalSciences)May2009Vo.l44No.3,1.5(AAS)(CS)(EAAI)[7]AAS=(mg/g)/FAO(mg/g)CS=(mg/g)/(mg/g)EAAI=(t/st/st/s)-n,n,t,s22.111.28%,2.21,,(70.71.0)g/100g,,()(20.81.1)g/100g,,,70.9%[9],1g/100g[8][8][9]20.81.126.631.434.070.71.068.029.451.42.32,14(2),8,,()FAO/WHO,EAA/TAA40%,EAA/NEAA0.60[10],EAA/TAA49.65%,EAA/NEAA0.99,,,()13.22g/100g,25.52%,2g/100g()[8][8][8](Asp)6.400.083.905.026.77(Glu)7.250.179.398.3010.73(Ser)3.250.462.322.152.82(His)5.220.142.341.761.96(Gly)2.650.082.422.794.14(Thr)*3.560.212.572.413.03(Ala)3.140.333.673.254.43(Lys)*4.240.235.544.596.49(Tau)2.140.13---(Met)*1.800.071.671.451.82(Val)*7.100.252.852.723.96(Phe)*6.810.422.492.352.94(Ile)*3.590.162.812.623.31(Leu)*2.530.295.024.415.77(Trp)*-0.760.730.75TAA59.6856.4653.0569.44EAA29.6322.8121.2728.07(EAA/TAA)/%49.6540.4140.1040.42EAA/NEAA0.990.680.670.68TAA:;EAA:;NEAA:;*:;-2.4(AASCSEAAI)3EAAIFAO/WHO111.14,84.69,(126.30,90.91[7]),,,,AASCS,,,,,3FAO/WHOmg/gFAO/WHOAASCSThr50.364053.9125.9093.43Lys59.985564.9109.0592.42Met+Cys25.503551.272.8649.85Val100.445057.6200.88174.39Phe+Tyr96.346095.5160.57100.88Ile50.794052.4126.9896.93Leu35.797084.151.1342.56Trp-1016.2---640()200954432.54,,,();,201,61,,;21,,,;;,4mg/100g()[9][9][9]1434.12521.16522.39809.68594.594938.06407.79280.22204.19507.72857.0076.2411.1929.03216.22478.7356.51108.2161.2938.612.261.210.410.230.5814.242.5425.823.524.2545.069.6316.424.527.721.861.23-0.100.150.050.090.010.040.060.180.02---0.613.660.900.500.50-,,,,,,,[1],.[J].,2002,27(5):381[2].[J].,2007,26(6):355[3],.[J].,2004,22(3):210[4]YangQ,ZhangXL,MaM,eta.lNewhigh-performanceliquidchromatographicmethodforsensitivedeterminationofpheomelanininbiologicalmaterialsbyprecolumnfluo-rescencederivatizationwithnaphthalene-2,3-dicarboxalde-hyde[J].JChromatogrA,2007,1146(1):23[5]IzgiB,GucerS,JacimovicR.Determinationofseleniumingarlic(Alliumsativum)andonion(Alliumcepa)byelec-trothermalatomicabsorptionspectrometry[J].FoodChem,2006,99(3):630[6],.[J].,1988,18(2):69[7],.[M].:,1984.[8],,.(2002)[M].:,2002.[9],,,.[J].,2007,28(4):32[10]Energyandproteinrequirements.ReportofajointFAO/WHOadhocexpertcommittec[R].Rome:FAOMeetRepSer,1973,52:40(2008-04-03)白雪洋,李凌#,孙国举,田丽晓,董文杰,陈雅丽450052#,,1960