晚上刘宇典本科毕业论文石广亮修改版2韩璐璐519

东北农业大学学士学位论文学号：A06090014大青叶多糖提取工艺研究学生姓名：刘宇典指导教师：石广亮所在学院：动物医学学院所学专业：动物药学研究方向：中兽药学东北农业大学中国·哈尔滨2013年5月NortheastAgriculturalUniversityundergraduatethesisStudentI.D:A06090014StudyonExtractionProcessofPolysaccharidefromFoliumIsatidisNameofstudent：LiuYudianGuidanceteachers：ShiGuangliangHostfaculties:AnimalVeterinarianAcademyInstituteSpecialties:VeterinaryPharmacyResearchdirection:ClinicalveterinarymedicineNortheastAgriculturalUniversityHarbin·ChinaMay·2013大青叶多糖提取工艺研究I摘要大青叶为十字花科植物菘蓝的干燥叶，为我国大宗使用药材，其制剂生产销售堪称中药之最，是清热解毒药的代表。本文以大青叶为实验材料，采用传统水提醇沉的方法提取大青叶多糖。选取提取时间、提取温度、提取次数和液料比四个因素，进行正交试验设计。利用重量法测定大青叶粗多糖的得率。以粗糖得率为指标优选工艺。最佳工艺为提取温度85℃、提取时间1.0h、提取次数2次、液料比25:1。本研究可为工业化生产和开发新的中兽药药源提供理论依据。关键词：大青叶多糖；提取；正交试验大青叶多糖提取工艺研究IIStudyonExtractionProcessofPolysaccharidefromFoliumIsatidisAbstractIsatisindigoticaFort.(IIF)isatwo-year-oldcruciferousvegetable.Itsroot,leaveandstemarenamedRadixIsatis(RI)andFoliumIsatis(FI),respectively.TheyarethecommontraditionalChinesemedicinewithsimilarefficacyandrepresentativemedicinesusedforrelievingheatandtoxic.Theextractionprocessofpolysaccharidesfromresiduestargetsforrawpolysaccharide,whichunderconditionsthatadding1%complexenzymesintomaterialsandextractingfortwiceand0.5hourspertimeattemperatureof80℃。Theextractionprocesswhichtargetsforfinepolysaccharideisunderconditionsthat25:1(V/M)andextractingfortwiceand1.0hourspertimeattemperatureof85℃。ThisresearchcanprovideatheoreticalbasisforutilizationandprocessimprovementofFI.Keywords:Foliumpolysaccharide;extract;Orthogonaldesign大青叶多糖提取工艺研究III目录摘要...................................................................................................................................................IAbstract............................................................................................................................................II目录.................................................................................................................................................III1前言..........................................................................................................................................-1-1.1大青叶研究概况...............................................................................................................-1-1.1.1来源及其药材性状.....................................................................................................-1-1.1.2在兽医临床上的应用......................................................................错误!未定义书签。1.2大青叶的研究现状............................................................................................................-1-1.2.1化学成分研究.............................................................................................................-1-1.2.2药理作用研究.............................................................................................................-1-1.3多糖的提取方法................................................................................................................-2-1.3.1溶剂提取法.................................................................................................................-3-1.3.2稀酸提取.....................................................................................................................-3-1.3.3碱提取.........................................................................................................................-3-1.3.4酶提法.........................................................................................................................-3-1.3.5超滤法.........................................................................................................................-3-1.3.6微波提取法.................................................................................................................-3-1.4本课题的研究意义........................................................................................................-3-2正交试验优选大青叶多糖的提取工艺........................................................错误!未定义书签。2.1材料与方法........................................................................................................................-4-2.1.1实验材料.....................................................................................................................-4-2.1.2实验仪器.....................................................................................................................-4-2.2大青叶多糖提取................................................................................................................-4-2.3各因素对多糖得率的影响....................................................................错误!未定义书签。2.3.1液料比对大青叶多糖得率的影响..............................................错误!未定义书签。2.3.2提取时间对大青叶多糖得率的影响.........................................错误!未定义书签。2.3.3提取温度对大青叶多糖得率的影响.........................................错误!未定义书签。2.3.4提取次数对大青叶多糖得率的影响.........................................错误!未定义书签。2.4数据处理方法........................................................................................错误!未定义书签。2.5正交试验优选大青叶中大青叶多糖水煮醇沉工艺研究...............................................-5-3结果与分析..............