花青素的提取-分离以及纯化方法研究进展

2008348(248)1113,,,,(,,,100083),,,,,(anthocyanins),,,,187922,22,6,(cyanindin,Cy)(pelargonidin,Pg)(delphin2idin,Dp)(peonidin,Pn)(petu2nidin,Pt)(malvidin,Mv)[1],150%12%12%12%7%7%(),27,72[2]1:()3(30771511),(2006BAD27B03),863(2007AA100405):2008-04-24,:2008-06-13,(glucose)(rhamnose)(ga2lactose)(xylose)(arabinose),[1]6,FOODANDFERMENTATIONINDUSTRIES1122008Vol134No18(Total248)1,,111(solventextraction),,,[3],,[4],,(48)(25)1%HCl1620h,015%1%,424h[6],1%HCl[1,4],[1](5070)12h[7,8],[7,8]112(pressurizedsolventextraction,PSE),(pressurizedliquidextraction,PLE)(acceleratedsolventextraction,ASE),,PSEArapitsas(2008)[9],:215g,99,7min,V()V()V()=9451113(extractionassistedbyhighhydrostaticpressure)2008Corrales[10],,(70),(600MPa)50%,3,114(microwaveassistedextraction,MAE)1986Ganzleret[11]2007,Sun[12]:115mol/LHCl95%(1585),5mL1g,53min,71,2,,36319g/g115(ultrasoundassistedextrac2tion,UAE)2007Chen[5],:41(mLg),200s,400W2008,Corrales[10],,(70),50%2004[13],V(0.1%HCl)V(95%)=406014060(40Hz)30min,89145%116(extractionassistedbypulsedelectricfield),DNA2007Lpez[14],PEF,2008Corrales[10],(70)(600MPa),PEF2008348(248)113,PEF,,PEF[15](2006)PEF,PEF117(sub2criticalwaterextrac2tion,SWE),100374,,,[16]King[17](110160)(),CO2,,,2005Ju[18](subcriticalsul2furedwater,SSW),(100160)60%(50C,1h)ORAC(oxygenradicalabsorbancecapacity)(ORAC),2100C100110C,ORAC,2007Luque2Rodrguez[19](2)(superheatedliquidextraction),:018%HCl2(11),120C,30min,112mL/min,8MPa3711118(super2criticalfluidex2traction,SCFE)SCFE,,[20](2006)CO2,25MPa6045min125,,119,,[21],,,015%3050%pH510,33,pH618,l4,810mg/g,120min11102(extractionassistedby2radiation)Ayed[22](2000)2,6kGy,2000mg/kg(SO2),6015min,1111,2003Cai[23]2,:624W,60s,40s,16,pH=5102005[24],CO2,,:30kHz,2%H2SO4,40min,502FOODANDFERMENTATIONINDUSTRIES1142008Vol134No18(Total248),211(paperchromatograph,PC),V()V()V()=415,V()V(2mol/LHCl)=11,V()V(HCl)V()=15382,1%,V(HCl)V()=397,,,(),212(thinlayerchromatograph,TLC),Harborne[25](1958),V()V()V(2mol/LHCl)=85692004Heidari[26](PTLC),(MerckCorp1),V()V(HCl)=11,0101%HCl,34C,(17401113158)mg/L(232)213(columnchromatograph,CC)(polyamide)(PVP),AmberliteXAD27,AmberliteXAD24,Sep2PakC18;AmberliteCG250,ToyopealHW240F,DiaionHP220,Dowex50W2X8;SephadexLH220,SephadexG225,Tatsuzawa(2008)[27]5%,DiaionHP220(malcolmiamaritima),3214(highperformanceliquidchromatog2raphy,HPLC)(HPLC),2005,Byamukama[6]2(RubuspinnatusRubusrigidus)2cyanidin32(62O2a2rhamnopyranosyl22glucopyranoside)cyanidin232O22glucopyrano2side2007Rentzsch[28]252carboxy2pyranocyanidin32O2(2G2glucosylrutinoside)52carboxy2pyranocyanidin32O2ruti2noside2151960Woo[29],,0111%()Patil[30](2007)(osmoticmembranedistillation,OMD),,,,40g/mL918mg/mL216(high2speedcountercurrentchro2matography,HSCCC/HPCPC)22003,Schwarz[31];2004,Du[32]2232O2(delphinidin232O2sambubioside)232O2(cyanidin232O2sambubioside),500mg,130mg232O277mg232O2,217(matrixsolidphasedispersion2extraction),(C18C8),,2008348(248)115,,,2006,Manhita[33],218Bridle[34](capillaryzonee2lectrophoresis,CZE),Watanabe[35](1998)(MEKC)4cyanidin232sambubioside252glu2coside,cyanindin232glucoside252guciside,cyanindin232sambubioside,cyaniding232glucoside219[36]:60%,607020min;,,,,4h;,3040;2,12h;,50%(60),3040,,50%,,,21102007,Lopes[37](tonsilterrana580FF)AmberliteXAD2721%,,,22,Andersen[38],3(Geraniumsylvaticum)5Andersen[39](Fragariaananassa)52carboxypyranopelargonidin32O22glucopyranoside3,(),,,,,,,,,,1Escribano2bailnMT,Santos2buelgaC,Rivas2gonzaloJC1Anthocyaninsincereals[J].RevJChromaogrtA,2004,1054:1291412MazzaG,MiniatiE1VegetablesandGrains[M],London:CRCPress,19933KjellT,vindMA1Colorstabilityofanthocyaninsina2queoussolutionsatvariousphvalues[J].FoodChem,2005,89:4274404MarkakisP1Anthocyaninsasfoodcolors[M].SanDiego:AcademicPress,1982.1632075ChenF,SunYZ,ZhaoGH,etal.Optimizationofultra2sound2assistedextractionofanthocyaninsinredraspber2riesandidentificationofanthocyaninsinextractusinghigh2performanceliquidchromatography2massspectrom2FOODANDFERMENTATIONINDUSTRIES1162008Vol134No18(Total248)etry[J].UltrasonSo,2007,14:7677786ByamukamaR,KiremireBT,AndersenM,etal.Antho2cyaninsfromfruitsofRubuspinnatusandRubusrigidus[J].JFoodCompAnal,2005,18:5996057,1[J].,2006,14(1):34358,,1[J].,2007,26(3):1961989ArapitsasP,TurnerC1Pressurizedsolventextractionandmonolithiccolumn2HPLC/DADanalysisofanthocyaninsinredcabbage[J].Talanta,2008,74,(5):1218122310CorralesM,ToepflS,ButzP,etal.Extractionofan2thocyaninsfromgrapeby2productsassistedbyultrasonicshighhydrostaticpressureorpulsedelectricfields:Acomparison[J]1InnovFoodSciEmergTechnol,2008,9:859111GanzlerK1Microwaveextraction:anovelsampleprepara2tionmethodforchromatography[J].JChromatogr,1986,371(11):29930612SunYZ,LiaoXJ,WangZF,etal.Optimizationofmi2crowave2assistedextractionofanthocyaninsinredrasp2berriesandidentificationofanthocyaninofextractsusinghigh2performanceliquidchromatography2massspec2trometry[J].Eur1FoodResTechnol,2007,225(34):51152313,,1[J].,2004,16(3):40440814LpezN,PurtolasE,CondnS,etal.EffectsofpulsedelectricfieldsontheextractionofphenoliccompoundsduringthefermentationofmustofTempranillograpes[J],InnovatFoodSciE