蛋白质组学——20141130版

蛋白质组学及其应用中山医学院蛋白组学实验室胡坤华ThesamegenesdifferentmorphologiesWhy？DNARNA蛋白质降解生命活动的直接执行体DNAmRNA蛋白质转录翻译基因蛋白质细胞特异性基因表达生理状态蛋白质相互作用温度应激状态培养条件药物作用数量有限结构相对稳定复杂性多变性直接反映生命现象复杂的调控1994年，澳大利亚Williams提出蛋白组学，研究不同时空状态蛋白质整体变化。蛋白质组蛋白质组（proteome）一种细胞、组织或完整的生物体所表达的全套蛋白质。蛋白质组学（proteomics）从整体水平研究细胞内蛋白质的组成及其变化规律。表达量变化、修饰水平变化、蛋白相互作用变化，揭示蛋白功能。6蛋白质组学传统方法：单一、已知、低效率蛋白质组学：整体、源头、高通量、创新蛋白质组学是前沿交叉学科•前沿性——国际热点(中国人类蛋白质组计划)•交叉性——原始创新机会•共享性——创新性技术平台，辐射多学科蛋白质组学特点•技术共享（涉及蛋白质均可）•周期短（1个月）•毕业、晋升论文的较好选择中山医学院蛋白质组学实验室1、Y.Li,C.Liu,Y.Zhao,K.Hu,J.Zhang.Sevofluraneinducesshort-termchangesinproteinsinthecerebralcorticesofdevelopingrats.ActaAnaesthesiolScand;57:380–390.20132、YanZheng,YongbinLin,DongrongSitu,LongJiang,XiaodongSu,HaoLong.AnalysisofDifferentlyExpressedProteinsInvolvedinMetastaticNicheoflung.TianjinLungCancerInstitute.20133、ZhengM,HuK,LiuW,LiH,ChenJ,YuX.ProteomicanalysisofdifferentperiodexcretorysecretoryproductsfromClonorchissinensisadultworms:molecularcharacterization,immunolocalization,andserologicalreactivityoftwoexcretorysecretoryantigens-methionineaminopeptidase2andacidphosphatase.ParasitolResMar;112(3):1287-97.20134、L.Deng,H.-L.Jia,C.-W.Liu,K.-H.Hu,G.-Q.Yin,J.-W.Ye,C.-H.He,J.-H.Chen,Y.-P.Xie,R.Dang,L.Zhang,N.-Y.Liu,L.ZhuandL.Zhang.Analysisofdifferentiallyexpressedproteinsinvolvedinhand,footandmouthdiseaseandnormalsera.ClinMicrobiolInfect,10.1111/j.1469-0691.03836.x,20125、DaohuWang,HuiLiang,XiaopengMao,WeiLiu,MingtaoLi,ShaopengQiu.Changesoftransthyretinandclusterinafterandrogenablationtherapyandcorrelationwithprostatecancermalignancy.TranslOncol.2012;5(2).6、GaoX,PangJ,LiLY,LiuWP,DiJM,SunQP,FangYQ,LiuXP,PuXY,HeD,LiMT,SuZL,LiBY.Expressionprofilingidentifiesnewfunctionofcollapsinresponsemediatorprotein4asametastasis-suppressorinprostatecancer.Oncogene..2012Aug12;29(32):4555-66。实验室服务发表文章7、JunPang,WeipengLiu,XiaopengLiu,LiaoyuanLi,YouqiangFang,QipengSun,ShaojunLiu,MingtaoLi,ZulanSu,XinGao.ProfilingproteinmarkersassociatedwithlymphnodemetastasisinprostatecancerbyDIGE-basedproteomicsanalysis.JProteomeRes.2010;9:216-226.8、Yan-XinLuo,JiCui,LeiWang,,Dian-KeChen.Identificationofcancer-associatedproteinsbyproteomicsanddownregulationofb-tropomyosinexpressionincolorectaladenomaandcancer.ProteomicsClin.Appl.20099、JingLuo,JineYang,Bing-YunYu,WeiLiu,MingtaoLi,Shi-MeiZhuang.IdentificationofSiah-interactingproteinasapotentialregulatorofapoptosisandcurcuminresistance.Oncogene.2010;29(48):6357-636610、LiuW,ZhouXW,LiuS,HuK,WangC,HeQ,LiM.Calpain-truncatedCRMP-3and-4contributetopotassiumdeprivation-inducedapoptosisofcerebellargranuleneurons.Proteomics.2009Jul;9(14):3712-28.11、WangJ,WuS,JinX,LiM,ChenS,TeelingJL,PerryVH,GuJ.Retinoicacid-induciblegene-ImediateslatephaseinductionofTNF-alphabylipopolysaccharide.JImmunol.2008Jun15;180(12):8011-9.12、KangL,YangZL,LiuW,ZhangLJ,LiuSJ,HuangMJ,LiMT,WangJP.(2008)SerumproteomicvariationstudyinpatientswithCrohndisease.ZhonghuaWeiChangWaiKeZaZhi.2008May;11(3):266-9.13、FuH,DouJ,LiW,LuoJ,LiKC,LamCS,LeeNT,LiM,HanY.Mecamylaminepreventsneuronalapoptosisinducedbyglutamateandlowpotassiumviadifferentialanticholinergic-independentmechanisms.Neuropharmacology.2008Mar;54(4):755-65.14、HongjunFu,WenmingLi,JialieLuo,NelsonT.K.Lee,MingtaoLi,KarlW.K.Tsim,YuanpingPang,MoussaB.H.YoudimandYifanHan.(2008)Promisinganti-Alzheimer’sdimerbis(7)-tacrinereducesβ-amyloidgenerationbydirectlyinhibitingBACE-1activity.BiochemBiophysResCommun.2008Feb15;366(3):631-615、MaC,YingC,YuanZ,SongB,LiD,LiuY,LaiB,LiW,ChenR,ChingYP,LiM(2007)dp5/HRKisac-Juntargetgeneandrequiredforapoptosisinducedbypotassiumdeprivationincerebellargranuleneurons.JBiolChem282:30901-3090916、Mei,Y,Yuan,Z,Song,B,Li,D,Ma,C,Hu,C,Ching,YP,Li,M.ActivatingTranscriptionalFactor3up-regulatedbyJNK/c-Juncontributestoapoptosisinducedbypotassiumdeprivationincerebellargranuleneurons,Neuroscience.2007,17、WangW,YangY,YingC,LiW,RuanH,ZhuX,YouY,HanY,ChenR,WangY,LiM.(2007)Inhibitionofglycogensynthasekinase-3betaprotectsdopaminergicneuronsfromMPTPtoxicity.Neuropharmacology52:1678-168418、YuanZ,MeiY,ZhouJ,TanM,SongB,MaC,YingC,LiD,ChingYP,LiM.(2007)E2F1isnotessentialforapoptosisinducedbypotassiumdeprivationincerebellargranuleneurons.NeurosciLett424:155-159.19、GuoQC,ShenJN,JinS,WangJ,HuangG,ZhangLJ,HuangG,YinJQ,ZouCY,LiMT(2007)ComparativeproteomicanalysisofhumanosteosarcomaandSV40-immortalizednormalosteoblasticcelllines.ActaPharmacolSin.28:850-858.20、LiW,PiR,ChanHH,FuH,LeeNT,TsangHW,PuY,ChangDC,LiC,LuoJ,XiongK,LiZ,XueH,CarlierPR,PangY,TsimKW,LiM,HanY*(2005)NoveldimericAChEinhibitorBis(7)-tacrine,butnotDonepezil,preventsglutamate-inducedneuronal21、LiC,CarlierPR,RenH,KanKK,HuiK,WangH,LiW,LiZ,XiongK,ClementEC,XueH,LiuX,LiM,PangY,HanY*(2007)Alkylenetether-lengthdependentg-aminobutyricacidtypeAreceptorcompetitiveantagonismbytacrinedimmers.Neuropharmacology.52:436-443.22、LiW,XueJ,NiuC,FuH,LamCS,LuoJ,ChanHH,XueH,KanKK,LeeNT,LiC,PangY,LiM,TsimKW,JiangH,ChenK,LiX,HanY*(2007)SynergisticNeuroprotectionbyBis(7)-tacrineviaConcurrentBlockadeofN-Methyl-D-aspartateReceptorsandNeuronalNitricOxideSynthase.MolPharmacol71:1258-1267.23、XiL,