PBS缓冲液的配制

PBS缓冲液的配制许志良，于尖峰海洲制药，2012/2/21PBS是最普遍不过的实验室缓冲液，但其配方各异。以下是总结：母液的配制：0.2MNa2HPO4：称取71.6gNa2HPO4-12H2O，溶于1000ml水0.2MNaH2PO4：称取31.2gNaH2PO4-2H2O，溶于1000ml水各种浓度PBS(pH=7.4)的配制：先配0.2MPBS(pH=7.4，100ml)：取19ml0.2mol/L的NaH2PO4，81ml0.2mol/L的Na2HPO4,即可。然后只需将0.2MPBS(pH=7.4)按相应比例适当稀释即可，如：0.1MPB（PH=7.4）：取500ml0.2MPBS，加水稀释至1000ml即可。0.01MPB（PH=7.4）：取50ml0.2MPBS，加水稀释至1000ml即可。0.02MPB（PH=7.4）：取100ml0.2MPBS，加水稀释至1000ml即可。若需要NaCl的话，加入NaCl至0.9%（g/100ml）即可。另：其它各种另PH值的0.2MPB（100ml)配方：pH0.2MNaH2PO4（ml）0.2MNa2HPO4（ml）5.793.56.55.89285.990106.087.712.36.185156.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851496.945557.038627.133677.228727.323777.419817.516847.613877.710.589.57.88.591.57.97938.05.394.7