Milliplex-Luminex-平台介绍(en)

ApplicationofMultiplexinginIVDManufactoryGraceYuanInstrumentFieldApplicationScientistMerckMilliporeToexplorebio-markerandinvitrodiagnosticOutlineLuminexxMAPtechnologyMuseCellAnalyzerDirectDetectSpectrometerOutlineLuminexxMAPtechnologyMuseCellAnalyzerDirectDetectSpectrometerAbconjugatedtomicrobeadsassuspensionarrays”Antibodiesinasandwichimmunoassay(ELISA)Abimmobilizedonchipsasplanararrays”Milliplex:highthroughputELISAtubes96wellplatemicroarrayMultiplexingThehistoryofproteinanalysistechnologySolution-phasekineticsSolid-phasekineticsSinglePlexAssaysMultiPlexAssaysInsulinLeptinGLP-1(A)Addsamples,stds,QCsandmatrixAddBiotinylatedSecondarycocktailAddstrep-PEWashWashLuminex200MilliporeEndocrine:Insulin,LeptinandGLP-1(active)xMAPBiomakersscreeningDetectionprincipleIdentificationofmicroshperesQuantificationRedlaser(635nm)Greenlaser(532nm)鞘液532nmAPDAPDAPD635nmPMT66.5um磁珠Core3.5%Fe3O4CoatingLayerCOOHSamechemicalsusedinmagneticbeadconjugationtobio-moleculesxMAP:ColorcodingofbeadsxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcodingxMAPtechnology-colorcoding18100specificmicrospheresDyeingprocesscreatesunique“spectraladdress”•Dyeratio•100beadcolors(regions)110010IL-636IL-1b9220xMAPApplicationTheapplicationofxMAPtechnologycoversimmunology,nuclearacid,enzymology,reactionofreceptorandtheligand.MilliplexPortfolioOfferings:•Inflammation/ImmunologyCytokine,SolubleCytokineReceptors,Th17,CD8+,MMP,TIMP•ImmuneResponseImmunoglobulinIsotyping,Sepsis•MetabolismAdipokine/Adipocyte,MetabolicHormone,LiverProtein,GutHormone,Pituitary,StressHormone,SpeciesSteroid/ThyroidHormone,IGFBindingProtein•CardiovascularCVD,Apolipoproteins,AcutePhase•BoneMetabolism•SkinPanel•CancerBiomarkerCirculatingCancerBiomarker,HumanAngiogenesis/GrowthFactor,Cancer/MetastasisBiomarker•NeuroscienceNeurodegenerativeDisease,NeurologicalDisorders,Neuropeptide•ToxicityKidneyToxicity,VascularInjury•CellularMetabolismAssaysPyruvateDehydrogenase,FattyAcidOxidation,OxidativePhosphorylation,OxidativeStress•StemCellAssays•CellSignaling/Epiquant21SA-PEPre-wetfilterplateAddbeadsAddsample&controls(samplepreparedincelllysisbuffer,serumdiluent)AdddetectionantibodyAddstreptavidin-PEReadplateInstantDataAnalysis!30min-O/N30min10min~40minTheexperimentalprocessxMAPTechnologyMultiplex：Multipletestingsolutionswithasmallamountofsample.Highspeed：10000test/hour.Lowcost：Savecostwhendetectingofmorethan3factors.Highsensitivity：Lowestlimitationis0.04pg/ml。Reproducibility：accurateandstableresults。Broadlinearrange：Detectionrangecovers10e5concentrationrange,providingaccurateresultsofdifferentsamplesatthesametime.NumberofplatesrequiredTotaltimetoresultResultsperplateTotalsampleusedperpanelInternalcontrolspossible?DynamicRangeLowerlimitofdetectionxMAP®TechnologyELISA3hours80010hours80YES50ml4ml1-10,000pg/ml~1pg/mlNO10-2500pg/ml~1pg/ml10proteins40samplesComparisonbetweenxMAPtechnology&ELISA25xMAPtechnologyapplication—ImmunologyMultiplexdetectionservesasthemostauthenticandaccuratetoolsforanimalmodelresearch.Results：AftertreatedwithLPS,theexpressionofcytokinesfluctuatesalongwiththetime.DIAGNOSTICMARKERSFOREARLYDETECTIONOFOVARIANCANCERProf.GilMor,YaleUniversityandcollaboratorsincludingMilliporeAdvantages:•Earlydetectionofmalignanttumor•Multiplexdetectionbenefitsfortissuespecificity•Non-invasivesampling•MultipletissuescreeningxMAPtechnologyapplication—cancerKitsInstrumentsSoftwareServicesMerckMilliporeprovidingintegratedapplicationsolution28InstrumentsKISSMultiplexAssaysHumanPanelsAdipokineApoptosisBoneCancerBiomarkerCVDCytokineEndocrineGutHormonePituitarySkinSol.CytoReceptorRatPanelsAdipokineBoneCVDCytokineEndocrineGutHormonePituitaryStressThyroidMousePanelsAdipokineBoneCVDCytokineEndocrineGutHormoneSolubleCytokineReceptorCaninePanelsAdipokineCytokineEndocrine29Non-HumanPrimatePanelCytokineMilliplexdetectionfor7speciesNEW：pig、catSampleTypesCulturesupernatantSerumPlasmaTissuelysatesUrineBloodspotsGingivalCrevicularFluidNasalLavageFluidTearsCerebralSpinalFluidBroncho-AlveolarFluidSalivaVaginalSecretionsSampleVolumesRoutineprotocol=25-50ml/wellHavemodifiedlowvolumeprotocols-25ml,10ml,5ml,1mlAccuracy10%Intra-assayandinter-assayvariabilitySampleTypesandVolumesMilliplexfeatures•Speed/HighThroughput:Simultaneouslymeasuretheconcentrationofalargenumberofdifferentanalytes,allowingyoutodomorefaster,gainingearlyandcomprehensivedatasocriticaltoyourwork.•Accuracy:Generatesreal-timeanalysisandaccuratequantificationofthebiologicalinteractions.•Reproducibility:High-volumeproductionofxMAPmicrospheresallowsassaystandardizationthatsolid-phasedflatarrayscannotprovide.•LowSampleVolume:Withminimalhands-ontime,youcanscreenmorethan40analytesinasinglesampleusingaslittleas25μLorless.OutlineLuminexxMAPtechnologyMuseCellAnalyzerDirectDetectSpectrometerMuseCellAnalyzerHighlyintuitivetouchscreeninterface2012Germanreddotdesignaward!Utili