GAL4-UAS系统在转基因技术中的应用研究进展-李春峰

REVIEWARTICLESGAL4PUAS1,1,1,23(1.,400715;2.,400047):GAL4PUAS,,GAL4,GAL4GAL4(UAS)-GAL4PUAS:GAL4UAS-GAL4,;UAS-,,,GAL4UAS-,GAL4PUAS:GAL4PUAS;;:Q789:A:1004-311X(2006)01-0078-04StudyofGAL4PUASSysteminTransgenicTechnologyLIChun-feng1,XIAQing-you1,ZHOUZe-yang1,23(1.TheKeySericulturalLaboratoryoftheMinistryofAgriculture,SouthwestUniversity,Chongqing400715;2.ChongqingNormalUniversity,Chongqing400047)Abstract:GAL4PUASsystemisatransgenicsystem.Inthissystem,aspecialpromoterisusedtodirectexpressionoftheyeasttranscriptionalacti2vatorGAL4inaparticularpattern,andGAL4inturndirectstranscriptionoftheGAL4-responsive(UAS)targetgeneinanidenticalpattern.ThekeyfeatureoftheGAL4systemisthattheGAL4geneandUAS-targetgeneareinitiallyseparatedintotwodistincttransgeniclines.IntheGAL4line,theactivatorproteinispresent,buthasnotargetgenetoactivate.IntheUASline,thetargetgeneissilentintheabsenceofactiva2tor.WhentheGAL4andUASlinesarecrossed,thetargetgeneisturnedononlyintheprogenyofthecross.ItwasreviewedherethestudyandtheapplicationoftheGAL4PUASsystematpresent.Keywords:GAL4PUASsystem;transgene;geneexpression:2005-10-15;:2005-11-29:(:30400328);973(2005CB121000):(1971-),,,,:,E-mail:[e-mail];3:(1958-),,,,,:,E-mail:[e-mail],,,,,,:,[1];(heatshockpromotor,hsp)[2],,,,,,,hsp,GAL4PPUAS,GAL4(galactose-regulatedupstreampromoterele2ment,GAL),,UAS(upstreamactivesequence,UAS)GAL4UAS,1988,FischerJA,etal.[3]GAL4,GAL4UAS,GAL4PPUAS,,GAL4PUAS1GAL4PUASGAL4PUAS,GAL4,GAL4,GAL4UAS,UAS-UAS-,UAS,GAL4UAS,,,GAL4UAS-(1)1GAL4PUASGAL4PUAS:GAL4UAS-GAL4,,UAS-,,GAL4UAS-,GAL4,GAL4GAL4UASGAL4GAL4,,UAS-,GAL4,UAS161:7820062BIOTECHNOLOGYVol116,No11:78Feb12006UAS,,GAL4PUAS:UAS-GAL4,,,UAS,GAL4PUAS,(ablation),GAL4PUAS[4]:1),;2);3)1.1GAL4GAL4GAL4,,GAL4-GAL4,GAL4,GAL4;,--GAL4,,,GAL4,GAL4,,GAL4,-GAL4,,GAL41.2UAS-UAS,GAL4,UAS-GAL4-,UAS-,56UAS,GAL4UAS,,GAL4UAS-,GAL4,(1)GAL4UAS-,,UAS--GAL4(2)-GAL4,GAL4UAS-,UAS-[5]GAL4PUAS,,,UAS--,,,,,UAS-lacZGFP1.2.1UAS-lacZlacZ-,lacZUAS,UAS-lacZGAL4UAS-lacZ,-X-Gal,-,lacZ-,6h,,1.2.2UAS-GFPUAS-GFPAequoriavictoria(GFP)GFP27kDa,,,GFPGFP,GFP,GFPBrandAYehE,etal.GFPUAS-GFP[6],,GFPGFPUAS-GFP[7],1.2.3UAS-tau-GFPUAS-tau-GFP[7]Tau,TauGFP,,1.2.4UAS-yellowUAS-yellowCallejaM,etal.UAS-[8]Yellowy-,GAL4UAS-y+,y-UAS-y+,GAL4y+,y-y+,UAS-yellow,CallejaM,etal.27-GAL4,y+1.31.3.1FLPPFRTGAL4,,GAL4UAS-,FLPPFRT,UAS-[9]FLP(Flppase),FRTs(FLPrecombinationtargets),FLPFRTsUASFRT--FRT,,,,GAL4poly(A)FLP,polyA,hspFLP,FLP,FRT-poly(A)-FRT1.3.2,GAL4HalfonMS,etal.[10]CambridgeSB,etal.[11]UV,,,,hsp-GAL4,hspGAL4,,1-2min,,,HalfonMS,etal.[10]hsp-GAL4PUAS-lacZ,lacZ,,GAL4-VP16(caged)UAS-,GAL4-VP16,6--3,4-(6-nitroveratrylchloroformate),GAL4-VP16,UAS-,365nm9720062GAL4PUASUV,GAL4-VP16UASUV,GAL4-VP16,UAS-CambridgeSB,etal.GAL4-VP16UAS-Ubx,UVUbx[11]2GAL4PUAS2.1GAL4PUASGAL4PUAS,GAL4UAS-UAS-GAL4,,UAS,GAL4UAS-,,GAL4PUAS,(tetanustoxin),SusterML,etal.[12]UAS-tetanustoxin(),150GAL4,,,A(RicinA)RicinA,RicinAFRTUASricinAricinA,UAS-FRT-poly(A)-FRT-ricinA,FLP,ricinAGAL4PUAS-ricinA,,[13](headinvolutiondefective,hid)reaper,,GAL4PUSA,reaperGAL4UAS-reaperUAS-hid,,[14]2.2GAL4PUAS(Bonemorphogeneticprotein,BMP)(dorsoventralpattern),HuQ,etal.[15]UASBMP4mutBMP4,UAS-BMP4UAS-mutBMP4,wntwnt1-GAL4,,BMP4ZhaoB,etal.[16]C(humansurfactantproteinC,SPC)-globin(GL)16(herpessimplexvirusprotein16,VP16)NF-Bp65,SPC-GL-VP-GAL4SPC-GL-p65-GAL4,GAL4RU486(mifepristone,)(progesteroneligandbindingdomain,PRLBD),SPCPRLBDPRLBD,RU486,ZhaoB,etal.(humangrowthhormone,hGH)UAS-hGHGAL4UASRU486,[16],,,,,2.3,GAL4PUAS,ImamuraM,etal.[4]3(B.moriactinA3,BmA3)(fibroinlight-chain,FiL),piggyBac,BmA3-GAL4FiL-GAL4,UAS-GFPFiL-GAL4UAS-GFP,F1GFP,GAL4PUAS,BMP4Xvent-2,Xvent-2Har2tleyKO,etal.[17]CMV(,cytomegalovirus)Pax-6,GAL4PUAS,CMV-GAL4Pax-6-GAL4UAS-Xvent-2,Xvent-2ScheerN,etal.[18]hsp70,hsp70-GAL4;UASmycnotch1(notch1intra),myc-notch1intra-UASJohnsonAA,etal.[19]GAL4PUAS-GFP,30%GFP,GFP,GAL4PUAS,3,;,GAL4PUAS,,,FLPPFRTUV,,GAL4PUAS,,,,GAL4:GAL4;,GAL4,,GAL4PUAS,:[1]WilderE.EctopicexpressioninDrosophila[J].MethodsMolBiol.,2000,137:9-14.[2]WidlakW,BenedykK,VydraN,etal.Expressionofaconstitutivelyac2tivemutantofheatshockfactor1underthecontroloftestis-specifichst70genepromoterintransgenicmiceinducesdegenerationofseminiferousepithelium[J].ActaBiochimPol.,2003,50(2):535-541.[3]FischerJA,GinigerE,ManiatisT,etal.GAL4activatestranscriptioninDrosophila[J].Nature,1988,332(6167):853-856.[4]ImamuraM,NakaiJ,InoueS,etal.TargetedgeneexpressionusingtheGAL4PUASsysteminthesilkwormBombyxmori[J].Genetics.,2003,165(3):1329-1340.[5]PhelpsCB,BrandAH.EctopicgeneexpressioninDrosophilausingGAL4system[J].Methods,1998,14(4):367-379.[6]BrandA.GFPinDrosophila[J].TrendsGenet,1995,11(8):324-325.08161[7]YehE,GustafsonK,BoulianneGL.GreenfluorescentproteinasavitalmarkerandreporterofgeneexpressioninDrosophila[J].ProcNatlAcadSciUSA.,1995,92(15):7036-7040.[8]CallejaM,MorenoE,PelazS,MorataG.VisualizationofgeneexpressioninlivingadultDrosophila[J].Science,1996,274(5285):252-255.[9]McGuireSE,RomanG,DavisRL.GeneexpressionsystemsinDrosophi2la:asynthesisoftimeandspace[J].TrendsGenet,2004,20(8):384-391.[10]Half