Bacillussimplex利用啤酒废水产微生物絮凝剂及应用研究

1626201691671—1815201626-0140-07ScienceTechnologyandEngineeringVol.16No.26Sep．20162016Sci.Tech.Engrg.、Bacillussimplex112114710232471000BacillussimplexPS1。BacillussimplexPS1COD5000mg/L、KH2PO41g/L、K2HPO42.5g/L、pH7、2%、30℃、170r/min、36h。96.8%、、、、90%COD80%BacillussimplexPS1。X172B20165104110122231500008、1321022102251978—。。[e-mail]。、、、、1—4、、、、、56、、、57—9。10—13。。Kurane3Ｒhodococcuserythropolis。。14PseudomonasalcaligenesPS-2596.75%。15Penicilliumpurpuroge-numHHE-P7Aspergillusversico-lorHHE-A26、、、、。16Ｒhodococcuserythropolis。BacillussimplexPS117。、、、、、、、、。Bacillussimplex。11.1BacillussimplexPS117。5g10gNaC15g18g1000mLpH7.0～7.2121℃30min。5g10gNaCl5g1000mLpH7.0～7.2121℃30min。20gKH2PO42gK2HPO45gNH42SO40.2gNaC10.1g0.5g0.5gMgSO4·7H2O0.2gH2Ol000mLpH7.0～7.5。COD9914mg/LpH6.3。1.21.2.150mL250mL30℃170r/min24h2%100mL250mL30℃170r/min36h6000r/min30min。1.2.2250mL0.4g1%CaC123mL2mL95mL1mol/LNaOH1mol/LHClpH7.01min3min10min550nmOD550AB。EE=B－A/B×100%。1.2.3COD、、、、pH、。。1CODCOD2000mg/L、3000mg/L、4000mg/L、5000mg/L、6000mg/L、7000mg/L、8000mg/LpH7.0～7.530℃170r/min36h。2COD0g/L、2g/L、4g/L、6g/L、8g/L、10g/L。3CODN0N10.05g+0.125g+0.125gN20.1g+0.25g+0.25gN30.15g+0.375g+0.375gN40.2g+0.5g+0.5g。。4CODKH2PO42g/L+K2HPO45g/L100%0、25%、50%、75%、100%KH2PO4+K2HPO4P0P1KH2PO40.5g/L+K2HPO41.25g/LP2KH2PO41g/L+K2HPO42.5g/LP3KH2PO41.5g/L+K2HPO43.75g/LP4KH2PO42g/L+K2HPO45g/L。。5pHCODHCINaOHpH5、6、7、8、9、10。。6CODpH12h。1.2.4COD、、pH、L934BacillussimplexPS1。1.2.5PS12mL95mL、、、、1.2.20.4g95mL1.2.2。、COD、14126Bacillussimplex1、COD、COD=B－A/B×100%A、CODB、COD。1Table1SourcesandcharacteristicsofwastewaterCODCr/mg·L－1/NTU/2598848144211580325491647532393222171831222.1CODCODPS11。COD5000mg/LCODCOD5000mg/L86.2%2.89g/LCODCOD7000mg/LCOD。CODPS1CODCOD。COD5000mg/L。2.2。PS1。20g/L2～4g/L1CODPS1Fig.1EffectofbrewerywastewaterCODonflocculationproductionandcellgrowthofPS1。2PS1Fig.2EffectofcarbonsourcesonflocculationproductionandcellgrowthofPS12.3、、PS1。3N0N10.05g+0.125g+0.125gN20.1g+0.25g+0.25g24116PS1。3PS1Fig.3EffectofnitrogensourcesonflocculationproductionandcellgrowthofPS12.4、、。PS1。4PS1。KH2PO41g/LK2HPO42.5g/LP293.5%3.59g/L。PS1KH2PO41g/L+K2HPO42.5g/L。2.5pH5pHpH5～7pH7～8pH8～10。pH6～980%pH7～84PS1Fig.4EffectofphosphateonflocculationproductionandcellgrowthofPS190%pH69pH14。5pHPS1Fig.5EffectofmediumpHonflocculationproductionandcellgrowthofPS12.6PS1624h。PS124h36h94.4%48h84h75.1%。34126BacillussimplexPS1、36h。。6PS1Fig.6EffectofincubationtimeonflocculationproductionandcellgrowthofPS12.7BacillussimplexPS1COD、、pH、4L934234。2Table2FactorsandlevelsoforthogonaltestCODA/mg·L－1B/%pHCD/h14000256.53025000507.03636000757.5423ＲBacillussimplexPS1COD＞pH＞＞。4CODpH。3A2B1C2D3COD5000mg/L、KH2PO40.5g/L+K2HPO41.25g/L、pH7、42h。3KA2B2C2D2COD5000mg/L、KH2PO41g/L+K2HPO42.5g/L、pH7、36h。3Table3OrthogonaldesignandtestresultABCD/%1111172.42±1.952122285.95±2.043133375.34±2.174212393.86±2.425223191.69±2.266231287.75±2.297313280.25±2.108321376.17±1.829332186.92±2.21K1233.71246.53236.34251.03K2273.30253.81266.73253.95K3243.34250.01247.28245.37Ｒ39.597.2830.398.584Table4VarianceanalysisoforthogonaltestFPA852.372426.1892.430.000B26.59213.302.880.082C473.772236.8951.380.000D38.02219.014.120.03482.99184.61P＜0.010.01＜P＜0.050.05＜P＜0.10。A2B2C2D296.85%A2B1C2D393.82%BacillussimplexPS1A2B2C2D2COD5000mg/LKH2PO41g/L+K2HPO42.5g/LpH736h。2.8BacillussimplexPS1、、、、790%COD80%、、、、。441167Fig.7Flocculationeffectforwastewaters31BacillussimplexPS1KH2PO4K2HPO4。2BacillussimplexPS1COD5000mg/L、KH2PO41g/L、K2HPO42.5g/L、pH7、2%、30℃、170r/min、36h。96.8%。3BacillussimplexPS1、、、、90%COD80%。BacillussimplexPS1。1AhmadHＲajabAAzniIetal．ProductionandcharacterizationofabioflocculantproducedbyAspergillusflavus．BioresourceTechnolo-gy20131271489—4932OkaiyetoKNwodoUUMabinyaLVetal．CharacterizationofabioflocculantMBF-UFHproducedbyBacillussp．AEMＲEG7．In-ternationalJournalofMolecularSciences201516612986—130033TakedaMKuraneＲKoizumijNetal．AproteinbioflocculantproducedbyＲhdococcuservthropolis．AgriculturalandBiologicalChemistry199155102263—22644．KJ-10．201154951—955ZhangYBLiZLiuQYetal．Flocculatingpropertyandingredi-entanalysisofthemicrobialflocculant-producingstrainKJ-10．Chi-neseJournalofEnvironmentalEngineering201154951—9555GongWXWangSGSunXFetal．BioflocculantproductionbycultureofSerratiaficariaanditsapplicationinwastewatertreatment．BioresourceTechnology200899114668—46746AljubooriAHＲIdrisAAl-jouboryHHＲetal．Flocculationbe-haviorandmechanismofbioflocculantproducedbyAspergillusflavus．JournalofEnvironmentalManagement2015150466—4717LianBChenYZhaoJetal．MicrobialflocculationbyBacillusmucilaginosusApplicationsandmechanisms．BioresourceTechnolo-gy200899114825—48318．．200933525—528ZhangNYinHQinHMetal．Studyonapplicationofbiofloccu-lanttoimprovementofthickenedsludgedewaterabilityinmunicipalwastewatertreatmentplant．ChineseJournalofEnvironmentalEngi-neering200933525—5289．．2014831208—1214GuMYZhuJSongSQetal．Identificationandfunctionalprop-ertiesofanovelflocculantproducingbacterium．ChineseJournalofEnvironmentalEngineering2014831208—121410YangMLiangYDouYetal．Isolationandidentificationofabioflocculant-producingstrainandoptimisationo