EffectsofCdcontaminationl

3113312(1,310029;2,330045),Cd.,CNCd,Cd,.CN,.Cd:.Cd.Cd,;Cd.Cd;.,Cd.Cd1001-9332(2005)11-2162-06X17115AEffectsofCdcontaminationonpaddysoilmicrobialbiomassandenzymeactivitiesandricephysiologicalin2dices.ZENGLusheng1,LIAOMin1,HUANGChangyong1,LUOYunkuo2(1DepartmentofResourcesScience,CollegeofEnvironmentalandResourceSciences,ZhejiangUniversity,Hangzhou310029,China;2CollegeofLandResourcesandEnvironment,JiangxiAgriculturalUniversity,Nanchang330045,China).2Chin.J.Ap2pl.Ecol.,2005,16(11):21622167.ApotexperimentwithriceundersubmergedconditionshowedthatwiththeincreaseofCdconcentration,soilmicrobialbiomasscarbon(Cmic)andnitrogen(Nmic)increasedinitiallybutdecreasedatacertainconcentration,andtheturningpointsvariedwithdifferentsoiltypes.SoilenzymeactivitieshadthesimilarvariationtrendwithsoilCmicandNmic,andtheturningpointsvariedwithdifferentsoiltypesandsoilenzymes.Thevariationcoefficientswereinorderofdehydrogenaseactivityacidphosphataseactivityureaseactivity.Soilrespirationrateandmetabolicquotientincreasedtardilywithincreasingcadmiumconcentration.Thechlorophyllcontentofricein2creasedinitiallybutdecreasedthenwiththeincreaseofCdcontamination,andtheturningpointsdifferedwithdifferentsoiltypes.Riceprolinecontentandperoxidaseactivitywereenhancedgraduallywithincreasingcadmi2umconcentration.Thevariationcoefficientsofricephysiologicalindicesonpaddysoilsderivedfromsiltyloamandclayedredearthwereinorderofperoxidaseactivitychlorophyllcontentprolinecontent,andperoxidaseactivi2typrolinecontentchlorophyllcontent,respectively.Correlationanalysisindicatedthattherewasaclosecorre2lationbetweenthevariationsofsoilmicrobialbiomassandenzymaticactivitiesandricephysiologicalindicesunderCdcontamination.KeywordsPaddysoil,Cdcontamination,Soilmicrobialbiomass,Soilenzymaticactivity,Ricephysiologicalindices.3(40201026,40371063)(2002CB410804).33.2005-01-20,2005-06-22.1CNPS,,[9]..,.,,[5].,Cd,,.Cd,,[18],,.Cd,[26].Cd2005111611CHINESEJOURNALOFAPPLIEDECOLOGY,Nov.2005,16(11)21622167,,2Cd2.,Cd,,2.2211()(),020cm,312mm,,1.510kg,80cm220cm,CdCl2215H2O,CK1381530mgkg-1(Cd)6,3.014gkg-1,014gkg-1,,15d.212.7,.,2d,40%3h,,,,251,.25d15d,4.2cm,15d()012gkg-1012gkg-1,3.1Table1CharacteristicsofthesoilusedSoiltypespH(H2O)TOC(gkg-1)Avail.P(mgkg-1)NTotalN(gkg-1)CEC(cmolkg-1)CdTotalCd(mgkg-1)CdAvail.Cd(mgkg-1)Sizecomposition(%)2100102mm010201002mm01002mmHS1)51971115539157110014159017430110665102818612HH2)5112291691016321561214701725010362215571020151)Paddysoilderivedfromsiltyloam;2)Paddysoilderivedfromclayedredearth.Thesamebelow.213,,,,.1,,222.,15d,30d.,010cm,,,,CN,.3,,,.214[11];Cd22,Cd011molL-1HCL,;C015molL-1K2SO4,TOC2500[24];N015molL-1K2SO4,[3];011molL-1NaOH[16];;;TTC[8].,;,;[28].215SPSS1115MicrosoftExcel2000.3311CdCN,CCd,(2).,1mgkg-1,,8mgkg-1;3mgkg-1,30mgkg-1.,15..,Cd.2N1mgkg-1.,CN,.,8mgkg-1,30mgkg-1.361211:2CdCNTable2EffectsofcadmiumtreatmentonsoilmicrobialbiomasscarbonandmicrobialbiomassnitrogenandmetabolicquotientMicrobialactivitiesSoiltypesCdCdtreatment(mgkg-1)CK1381530MeanCV(%)CHS67147b1)11012)75166a210768121b214857116c310446156d111938199e21845812521126Cmic(mgkg-1)HH895175cd16141924116b13167967151a14147924116b11101916191bc13190884157d161589181843122NHS20146b111723146a016621116b114319141bc019317195c014513146d11161913217113Nmic(mgkg-1)HH72151e210891156a111187141b115482123c110880183c112477122d1115811967199RespirationHS9108e01139151d01089182c011110103bc011910122b011610185a011391925186rate(CO22Cgkg-1h-1)HH5132e01075185d01155198cd01136115c01136141b01136169a011361077153MetabolicHS0114d010010114d010020114d010040118c010060122b010040128a01002011830134quotientHH01006c01006c01006c01007b01007b01008a01011197(h-1)1)LSD(P0105)ThedifferentlettersinthesamelineindicatesignificantdifferenceatP0105levelaccordingtoLSDtest,thesamebelow;2)Standarddeviation1Thesamebelow.N,.2,CN,,(2).Cd,,8mgkg-1.,15mgkg-1.Cd,,CN;NC.CN[25]..,CdCN,Cd.[6].Cd,,,Cd,,[14].CN,Cd[27]..,Cd,.,.,,,.,,[15].,CNC,C.,,pH().,,.,C,.,,,[1],,.312CdCd3.Cd,,,.8115mgkg-1,1533mgkg-1.2Cd,30mgkg-1.Cd.,2Cd:..,.,,.4612163CdTable3EffectsofcadmiumtreatmentonsoilenzymeactivitiesSoilenzymeactivitiesSoiltypesCdCdtreatment(mgkg-1)CK1381530MeanCV(%)1)HS0133c01010134c01010137ab01010138a01020137ab01010135bc010101366160UreaseHH0153c01020155bc01010156abc01010158ab01010159a01040158ab0102015641882)HS1103b01091132a01111116ab01121114ab01101113ab01081102b0111112015131AcidphosphataseHH2161c01153110b01083167a01162173c01072167c01112123d01082184161903)HS0112c01020112c01020115c01010120b01030124a01010115c0101011630106DehydrogenaseHH0118c01010131a01020133a01020126b01010125b01010123b01010126201381)Ureaseactivityunit:NH3+2Nmgg-1d-1,37;2)Acidphosphataseactivityunit:Phenolmgg-1d-1,37;3)Dehydrogenaseactivityu2nit:TPFmgg-1d-1,37.4CdTable4EffectsofcadmiumtreatmentonricepartphysiologicalindicesPhysiologicalindicesSoiltypesCdCdtreatment(mgkg-1)CK1381530MeanCV(%)HS4138c01164147c01135133a01135156a01114194b01194141c0111418510124Chlorophyll(mgg-1FW)HH4164bc01174176b01165133a01114166bc01084147c01144146c013141726180HS49147e018350156e014753166d015456166c0145591