MBR膜污染过程中微生物群落结构与代谢产物分析高大文

46220142JOUＲNALOFHAＲBININSTITUTEOFTECHNOLOGYVol.46No.2Feb．2014MBＲ高大文，辛晓东150090MBＲT－ＲFLPA/O－MBＲＲ1、Ｒ2pTMCake、．Ｒ130℃SＲT60d、FluxFM9.09L/m2·hDO4mg/L30dCakeOribacterium、Cytophagasp．、Anaeromyxobacter、Paracoccus、bp180ComamonadaceaeSaprospiraceae、Nitrospira、Thiothrixsp．bp92Ｒ220℃SＲT30dFM13.42L/m2·hDO2mg/L11dCakeAnaeromyxobacter、Oribacterium、SaprospiraceaeMyxobacteriumThiothrixEikelboomii、γ-Proteobacterium、Nitrospira、Thiothrixsp．bp52．．Cake．EPS、SMPCakeEPS．－X172A0367－6234201402－0026－07AnalysisofmicrobialcommunitystructureandmetabolitesduringtheMBＲmembranefoulingprocessGAODawenXINXiaodongStateKeyLaboratoryofUrbanWaterＲesourceandEnvironmentHarbinInstituteofTechnology150090HarbinChinaAbstractAimingatthemembranefoulingproblemproducedduringMBＲworkingprocessdifferentcontrollingoperationswereusedontwosetsofparallelA/O－MBＲsystemsＲ1andＲ2．ThechangingsituationofthemicrobialcommunitystructureduringthemembranepollutionprocesswasanalyzedbyT-ＲFLPmethodandthemicrobialmetaboliteswerequantified．TheresultsshowthatunderthedifferentoperationalconditionsthedominantmicrobialspeciesofCakelayerinＲ1systemcontrollingmethodtemperatureis30℃SＲTis60daysFluxis9.09L/m2·hDOis4mg/LisOribacteriumCytophagasp．AnaeromyxobacterParacoccusbp180andComamonadaceaerespectively．ThemembranefoulingcycleofＲ1reactoris30days．ThedominantmicrobialspeciesofCakelayerinＲ2systemcontrollingmethodtemperatureis20℃SＲTis30daysFluxis13.42L/m2·hDOis2mg/LisAnaeromyxobacterOribacteriumSaprospiraceaeandMyxobacteriumrespectively．ThedominantmicrobialspeciesofmembranewireinＲ1isSaprospiraceaeNitrospiraThiothrixsp．andbp92respectively．ThedominantmicrobialspeciesofmembranewireinＲ2isThiothrixEikelboomiiγ-ProteobacteriumNitrospiraThiothrixsp．andbp52respectively．ThemembranefoulingcycleofＲ2reactoris11days．ThedifferentrunningmethodsonA/O－MBＲsystemshaveasignificantinfluenceonthesuccessionofmicrobialcommunity．TheascendingofmicrobialdiversityShannonindexHofCakelayerandmembranewiremayacceleratethemembranefoulingprocess．TheobviousincreasingtrendofmicrobialmetabolitesEPSSMPcontentresultsinthemembranepollutiongettingworse．EPSoftheCakelayerhasadominantcontributiveeffectontheprocessofmembranefouling．KeywordsA/O-MBＲmembranefoulingmicrobialcommunitystructureEPSSMP2013－02－06．21177033．1967—[e-mail]tes9．．、A/O－MBＲ．11.1A/O－MBＲ1A/O－MBＲＲ1Ｒ2．8L．200～250r/minmin12∶3．PE0.4μm0.11m2．!#$%&’()1—2—3—4—5—6—7—8—9—．1A/O－MBＲ1.210CODCr295～335mg/LpH7.1～7.8NH4+－N37～42mg/L．3000～4000mg/L．1.3Ｒ1、Ｒ21．1SＲT/dθ/℃FM/L·m－2·h－1DO/mg·L－1Ｒ160309．094Ｒ2302013．422Ｒ1、Ｒ2pTM．Ｒ1、Ｒ23011d．1.41.4.1Ｒ1、Ｒ2、、CODCr、NH4+－N、NO2－－N、NO3－－N、DO、、pH．CODCr、NH4+－N、NO2－－N、NO3－－N11DO、、pHWTWpH/oxi340i．1.4.2pTMpTM0.04MPa．、．1.4.3EPS、SMPSMPEPS12．CakeSMP13．EPS－NaOH14SMP15．－EPS、SMP16BCAEPS、SMP．1.4.4Cake1～22cmCakeDNAEPS．DNADNAEPS1.4.3．1.5mL1mLPBS．PBS·72·2MBＲDNADNA．1.4.5T－ＲFLP、A/O－MBＲNCBIGenbank99%．T－ＲFLP1718．PCＲFAM8F1492ＲAfaI．19．22.1Ｒ1、Ｒ230d、20dCODNH4+－N2．Ｒ1、Ｒ2COD95%90%．A/O－MBＲ．2Ｒ1、Ｒ2/mg·L－1/mg·L－1/%Ｒ1CODCrNH+4－N316～35037～5211．582．3396．094．0Ｒ2CODCrNH+4－N303～34637～5113．581．3795．596．02.2Ｒ1pTM．1～6dpTM、2kPa/d．7～25dpTM200.89kPa/d．26dpTM30pTM0.04MPa2.5kPa/d．Cake．Ｒ21～4d4kPa/d．5～8dCake2kPa/d9～11CakepTM3kPa/d2．!!#!!$!!%!!&!&$’()&&&$&’&(&)%&%$%’%(%)!*+&+%#,-.-/02Ｒ1、Ｒ2pTM2Ｒ1Ｒ2MBＲ．2.32.3.1CakepTMＲ1、Ｒ2CakeT－ＲFLP3．!#$%&!#$’()*)!)!#)&&)&%)&$)*&)*+!,-’-./aＲ1!#$%&!#$’(!)*’*+,-.-/-&!-.-.0-./bＲ21—Orbacterium2—Deltaproteobacterium3—Variovoraxsp．4—Myxobacterium5—Cytophagasp．6—Anaeromyxobacter7—bp1808—Saprospiraceae9—Paracoccus10—bp20311—Comamonassp．12—Comamonadaceae13—UnculturedNitrospira．sp．14—Betaproteobacterium15—UnculturedXanthomonassp．3CakeT－ＲFLP·82·463pTM0.003MPaＲ1CakeOribacteriumDeltaproteobacteriumCake11Ｒ2CakeAnaeromyxobacterＲ26Ｒ1．pTM0.015～0.030MPaＲ1CakeCytophagasp．、Anaeromyxobacter、Paracoccus．．β、．．18868Ｒ2CakeOribacteriumSaprospiraceae30～37℃pH7、、．79．Ｒ2CakeＲ1．pTM0.037MPaＲ1CakeComamonadaceae21Comamonassp．5．Ｒ20.039MPaCakeMyxobacterium5．．Ｒ1CakeDeltaproteobacteriu、Anaeromyxobacter、Paracoccus、bp180、Cytophagasp．．pTM．Ｒ2CakeAnaeromyxobacter、Cytophagasp．、Oribacterium、Saprospiraceae、Variovoraxsp．Myxobacterium．Cake．CakepTMＲ1、Ｒ2pTMCake．Ｒ1Ｒ2．Ｒ1、Ｒ2pTMＲ20.820.81．CakeCake．2.3.2pTMＲ1、Ｒ24．!#$%&!#$’(!)*’*+,-.-/-!#-&$-.!-./-%aＲ1!#$%&!#$’(!)*’*+,-.-!-!#-&$-/-/0bＲ21—Saprospiraceae2—Thiothrixsp．3—ThiothrixEikelboomii4—Nitrospira5—bp586—UnculturedXanthomonassp．7—bp928—bp509—bp5210—γ-Proteobacterium4T－ＲFLPＲ1pTM0.003～0.010MPaSaprospiraceaeNitrospira．3332．Ｒ2ThiothrixEikelboomiiγ－ProteobacteriumγγNH4+、10～43℃．2324．Ｒ1Ｒ2．pTM0.01～0.03MPa·92·2MBＲＲ1ThiothrixEikelboomiiThiothrixsp．3626Ｒ2Nitrospirabp50AfaI50bp2327．Ｒ2Ｒ1．Ｒ1Nitrospirabp921417Ｒ2Thiothrixsp．bp521516．Ｒ1Ｒ2．Cake．Saprospiraceae、ThiothrixEikelboomiiNitrospiraＲ1．Thiothrixsp．NitrospiraＲ2．CakeＲ1Ｒ2．Ｒ1、Ｒ2pTMＲ20.620.74．Cake．2.3.3ＲＲ=ΔpJη=1Kη．ηPa·sΔpPapTM．ηＲm－1KL·m2·h－1·kPa－1ＲK－1．ＲpTMK－1pTM．ShannonHH=－∑pilnpii=12…S．piT－ＲFLPiST－ＲFLPpTM．CakeShannonHK－156．Pearsonrp－1.0～1.02．0＜|rp|≤0.30.3＜|rp|≤0.50.5＜|rp|≤0.80.8＜|rp|≤1．pPearson．!#$%#%!&$’()#*+$’(,,#-&$’.,,$&$’$$#!&$’!)$+$’.**#-&$’.!,$&$’$#.%#!)(*&/#0!12345/#46748995CakeShannon