包埋固定化微生物的硫自养反硝化实验研究欧阳丽华

32620116ENVIRONMENTALSCIENCEVol．32No．6Jun．2011*200240Na2S2O3220d、．N0.22kg/m3·d23dNO－3-N100mg/L．、NO－3HRT0.96kg/m3·dHRT1hNO－3-NTN90%～100%39.8mg/L·hHRT2h．NO－2-NS2－．．X172A0250-3301201106-1644-092010-07-152010-09-252008ZX07106-2-21985～[e-mail]*[e-mail]-BasedAutotrophicDenitrificationbyImmobilizedPelletsOUYANGLi-huaZHOUWei-liZHANGZhen-jiaWANGHuiSchoolofEnvironmentalScienceandEngineeringShanghaiJiaoTongUniversityShanghai200240ChinaAbstractAutotrophicdenitrificationbyimmobilizedmicroorganismswasinvestigatedinthisstudy．Usingthiosulfateastheelectrondonorthelabscaleup-flowsludgeblanketreactorrancontinuouslyfor220daysundertheroomtemperaturetoinvestigatetheeffectsofcultivateconditionsinfluencefactorsandoptimaloperatingparameters．Ittook23daystofinishtheinitialdomesticationstage．Anearlycompleteremovalofnitrate100mg/LwasattainedatnitrateloadingratesNLRof0.22kg/m3·d．TheresultsrevealedthatthedenitrificationefficiencywasdependentontemperatureinfluentnitrateconcentrationandhydraulicretentiontimeHRTandtemperaturewasthemostimportantfactor．Inspringandautumnnitrateandtotalnitrogenremovalefficiencyremained90%-100%attheNLRof0.96kg/m3·d．TheshortestHRTandthehighestdenitrificationratecouldachieved1hand39.8mg/L·hseparately．WhileinwintertheshortestHRTwas2handnonitriteorsulfideaccumulationwasdetected．Molecularbiologicalanalysisindicatedthatdenitrifyingbacteriawerere-enrichedsuccessfullyintheimmobilizedpellets．Keywordsnitrateautotrophicdenitrificationthiosulfateimmobilizationembedding、、C/N、、1．2．320℃NO－3-N99%HRT1.8hSSO2－4NO－3．Sierra-Alvarez4/100mg/LNO－3-N0.3kg/m3·dNO－2-NS2－．Vaiopoulou5S/N．Zhang6PVApH．Gadekar7NO－3-NS/NHRTSSO2－4S2－NO－3-N．89SCaCO3pH10～131415～17DOI:10.13227/j.hjkx.2011.06.0336181920．S2O2－3SNa2S2O3Na2SpH、、．11.188mm500mm3.2L1.6L．、、、、51．Na2S2O3nS2O2－3∶nNO－3=1∶1MgCl2·6H2ONaH2PO4NaHCO3．1．1Fig．1Setupofthecontinuousexperimentsystem1.2、NO－3、NO－2、NH+4、TN、S2－、SO2－421N2/O2GC-14BpH．1N100mg·L－1Table1CompositionofsyntheticwaterNaNO3Na2S2NO3·5H2OMgCl2·6H2OKH2PO4NaHCO3/mg·L－1607.141771.43631341.3－80℃DNA2223．12、SDS、RNaseA1min50℃1hNaAC15minDNATEDNA．PCRnirSnirK242PCRPCR25μL．2μLDNA400nmol/LTaqSC00102.5dNTP200μmol/L1×PCR1500μmol/LMgCl2．2nirS、nirKTable2PrimersforthedetectionofnitritereductasegenesnirKandnirS5'-3'/bpnirK1FGGA/C-ATG-GTG/T-CCC/G-TGG-CA526～542514nirK5RGCC-TCG-ATC-AGA/G-TTA/G-TGG1023～1040nirS1FCCT-AC/TT-GGC-CGC-CA/GC-AA/GT763～780890nirS6RCGT-TGA-ACT-TA/GC-CGG-T1638～16531.4NO－3-N546132HRT．220d、932526370mg/L40mg/L10mg/L、．B、C、D70、4010mg/LHRT．3Table3Operatingparameter/dt/℃NO－3-N/mg·L－1HRT/hNLR/kg·m3·d－1A0～2322～2810010±0.50.23±0.01B24～5115～23706～20.28～0.84±0.01C52～659～14406～20.16～0.48±0.01D66～1195.6～12106～0.50.04～0.5±0.01E120～14310～12106～40.04～0.06±0.01F144～16211～15103～10.08～0.24±0.01G163～18412～21406～10.16～0.96±0.01H185～19420～23101～0.620.24～0.37±0.02I195～22023～26401.5～10.64～0.96±0.0122.1NO－3-N220dAE2NO－3-N100mg/LHRT10h23dNO－3-N10mg/L9.2mg/L·h．10～12℃NO－3-N10mg/LHRT6hNO－3-N1.5mg/LNO－3-N85%40dHRT4hNO－3-N85%．NaHCO3NO－3-N1.5mg/LNO－3-N85%．NO－3-N70mg/L2BHRT6h4hNO－3-N3mg/LNO－3-N95%HRT3h2hNO－3-NHRT3hNO－3-N90%HRT2hNO－3-N10mg/L85%～90%．20℃NO－3-N70mg/LHRT2h0.83～0.85kg/m3·d．NO－3-N40mg/L2C、G、IC12℃G12℃21℃HRTI22℃．CNO－3-N2mg/L95%HRT2h0.48kg/m3·dGNO－3-N0mg/LHRT1.5h1hNO－3-NIG．NO－3-N40mg/LHRT2hHRT1h0.96kg/m3·dNO－3-N1mg/LNO－3-N100%．NO－3-N10mg/L2D、F、HD5～12℃HRT6h1hNO－3-N1mg/L98%．83dHRT0.5h8℃NO－3-N40%5～7℃HRT2h0.12kg/m3·dNO－3-N3mg/L70%．F11～15℃6461622dNO－3-N3mg/L85%153dHRT1hNO－3-N60%HRT1.5h．H20～23℃HRT1hNO－3-N100%HRT40min0.62～0.68h10dNO－3-N3mg/L80%．NO－3-N10mg/LHRT1h0.24kg/m3·d15℃HRT1.5hNO－3-N100%8℃HRT2h．2220d．3HRT、2728．2Fig．2VariationofcNO－3-Nincontinuousexperiments2.2NO－2-NNH+4-NNO－3-NNO－2-NNO－2-N15mg/L10dNO－3-NNO－2-N2mg/L．NO－2-NNO－2-N0.2mg/L．NH+4-N3NH+4-N1mg/LNO－3-NNH+4-N．3188dHRT40minNO－3-N3mg/LNO－2-NNO－2-N3Fig．3VariationofcNO－3-NcNO－2-NcNH+4-NincontinuousexperimentsHRT2～3mg/L20%NH+4-N．746132NO－2-NNO－2-NNO－3-NNO－2-N．HHRT40minNO－2-N0.36kg/m3·dIHRT1h0.96kg/m3·dNO－2-NNO－2-NHRTHRT1hNO－2-N．2.3TNNO－3-NTN．NO－2-NNH+4-NTN42NO－3-N．4TNFig．4VariationofcTNincontinuousexperiments2.4N2/O25N2/O2N2/O270mg/L40mg/LN290%585～161d8～12℃NO－3-N10mg/L185～194dN2/O2N285%N2N2NO－3-N．2.5S2－SO2－4nS2O2－3∶nNO－31∶1S2O2－3NO－3．6SO2－45N2/O2Fig．5PercentageofN2/O2inthebiogas6S2－SO2－4Fig．6VariationofcS2－andcSO2－4incontinuousexperiments200mg/LS2－SO2－4．SO2－4S2－SO2－4S2O2－3560mg/LSO2－4700mg/LS2O2－3320mg/LSO2－4400mg/LS2O2－380mg/LSO2－4250mg/L．SO2－4S2－SO2－4S2O2－3．SO2－4NO－371molNO－31.9molSO2－4．2.6220d8℃NO－3-N、HRTNO－3-NNO－3-N．846167SO2－4NO－3Fig．7CorrelationbetweenthecSO2－4andremovedcNO－315～28℃NO－3-N70mg/LHRT2hNO－3-N11mg/L85%NO－3-N40mg/LHRT1hNO－3-N1.5mg/L97%NO－3-N10mg/LHRT40minNO－3-N2mg/LNO－2-NTNHRT1hNO－3-N100%9～14℃NO－3-N40mg/LHRT1.5hNO－3-N1.5mg/L97%5～8℃NO－3-N10mg/LHRT2hNO－3-N2.86mg/L77%．33.1、NO－3-N．810mg/L、HRT1h、、．815～28℃9～14℃0.48kg/m3·d20mg/L·h．8℃0.12kg/m3·d4.27～5.54mg/L·h．．．8Fig．8Relationshipbetweenthetemperatureandnitrateremoval3.2HRT、HRT．2153～194d0.16kg/m3·d40mg/L、HRT6h10mg/L、HRT1.5h0.35～0.48kg/m3·d40mg/L、HRT2h100%10mg/L、HRT40minNO－2-NHRTHRT．3.3pHpHpH220dpHpH6.5