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J.LakeSci.2015273459-465http//.jlakes.org.E-mailjlakes@niglas.ac.cn2015byJournalofLakeSciences、Anabaenaflos-aquae*12323**12323123140004524007143400714、、.Lehman、Steele8PO3-4-P0、0.1、0.2、0.5、0.75、1.0、2.5、5.0μmol/L415、20、2530℃41000、2000、30005000lx.Monod20℃3000lxμmaxKS0.447d-10.081μmolP/LLehmanSteeleμmaxLehman21.22±0.98℃μmaxKS0.421±0.011d-10.055±0.009μmolP/LSteeleμmaxKS0.461±0.010d-10.051±0.009μmolP/LIk2650.93±88.19lx.ModelingofAnabaenaflos-aquaegrowthkineticsoflightintensityandtemperaturewithindifferentlevelsofphosphorusconcentrationsZHANGPing123LIZhe23WANGSheng1GUOJinsong23XIAOYan23&LIUJing1231FacultyofUrbanConstructionandEnvironmentalEngineeringChongqingUniversityChongqing400045P.R.China2ChongqingInstituteofGreenandIntelligentTechnologyChineseAcademyofSciencesChongqing400714P.R.China3KeyLabofReservoirWaterEnvironmentChineseAcademyofSciencesChongqing400714P.R.ChinaAbstractAnabaenaflos-aquaeisoneofthecommonharmfulcyanobacteriaspecies.Howeveritsgrowthkineticsco-impactedbyphosphateconcentrationlightintensityandtemperaturehaverarelybeenreported.InthispapereightPO3-4-Pconcentrationgra-dients00.10.20.50.751.02.5and5.0μmol/Lfourwatertemperatures152025and30℃andfourlightintensities100020003000and5000lxwereselectedtocarryoutsinglefactorbatchexperiments.Amaximumspecificgrowthrateμmaxwasusedtoindicatealgaespecificgrowthrateunderoptimalgrowthcondition.Half-saturationconstantKScouldbeappliedtodescribeaffinityofalgaetonutrients.RegressionresultsfromMonodmodelshowedthatAnabaenaflos-aquaereachedμmaxat20℃and3000lx.TheμmaxandKSwere0.447d-1and0.081μmolP/Lrespectively.RegressionresultsbasedonLehmanandSteeleModelthatexaminestherelationshipbetweenspecificgrowthrateandcontinuouschangingoftemperatureaswellaslightintensitydemonstratedthattheoptimumlightintensityIkandtemperatureToptwere2650.93±88.19lxand21.22±0.98℃respectively.BesidesthekineticparametersμmaxKSandToptfromLehmanModelwere0.421±0.011d-10.055±0.009μmolP/Land21.22±0.98℃respectively.IntheSteelemodelμmaxKSandIkwere0.461±0.010d-10.051±0.009μmolP/Land2650.93±88.19lxrespectively.KeywordsAnabaenaflos-aquaekineticmodellightintensitytemperaturephosphorus***5117921551309220KZCX2-XB3-14.20140616201410091989~E-mailzhangping890511@163.com.E-mailzheli81@sina.com.460J.LakeSci.20152731-2.“”3-4.Anabaenaflos-aquae5-6.、7.、.8.Robarts9、.10CO2、、.、、.、.MonodLehmanSteele“”“”.11.1FACHB-245.1.21.2.1BG-11pH7.5.K2HPO40、0.1、0.2、0.5、0.75、1.0、2.5、5.0μmol/L0、3.1、6.2、15.5、23.25、31.0、77.5、155.0μg/L.121℃30min.148h4200/min15min15mg/L33×105cells/ml11.1Tab.1Designofexperimentalvariables/℃/lxP11530002203000325300043030005201000620200072050000、0.1、0.2、0.5、0.75、1.0、2.5、5.0μmolP/L8250ml112h∶12h3~4.8001.0ml.2<5%50.1.2.2Monod8PO3-4-P0、0.1、0.2、0.5、0.75、1.0、2.5、5.0μmol/L12-1415、20、25、30℃41000、2000、3000、5000lx4.31.1.2.31Monod.Monod15-16.Monod17.μ=μmax·SKS+S1μd-1μmaxd-1Sμg/LKSμg/L、Anabaenaflos-aquae461.μmaxKS.Leneweaver-Burk18Monod2KS/μmax1/μmaxKSμmax.1μ=KSμmax·1S+1μmax2Monod、.LehmanSteele.LehmanSteele.3.2Lehman.LehmanμmT=μmax·SKS+Sexp-2.3Topt-T/T2m3μmTTd-1Topt℃T℃Tm℃..3Steele.Steele、.μmL=μmax·SKS+SIIkexp1-II()()k4μmLLd-1IlxIklx..22.1、、13、3~7d3~8d1μmolP/L.20℃3000lx5000lx、0~1.0μmolP/L6d2.5~5.0μmolP/L7d.2.22.2.1MonodMonod、μmax15、20、2530℃0.380、0.447、0.3840.355d-1μmax20℃2.KSμmax15、20、2530℃KS0.044、0.081、0.0400.021μmolP/L.1000、2000、30005000lx0.349、0.407、0.447、0.371d-1.μmax3000lxμmax.KSμmax1000、2000、30005000lxKS0.025、0.034、0.081、0.035μmolP/L462J.LakeSci.20152731、Fig.1DensityvariationsofAnabaenaflos-aquaeunderdifferenttemperatureandlightintensityconditions2.20℃3000lx.MonodPO3-4-P.2、MonodTab.2KineticparametersofMonodmodelunderdifferenttemperatureandlightintensityconditions/℃/lx/KS/μmax/1/μmaxR2μmax/d-1KS/μmolP/L1530000.1162.6320.7980.3800.0442030000.1802.2360.8920.4470.0812530000.1042.6030.8630.3840.0403030000.0592.8210.7180.3550.0212010000.0722.8620.7690.3500.0252020000.0832.4570.9600.4070.0342050000.0942.6980.8910.3710.0352.2.2LehmanLehman2.LehmanRSS=0.082R2=0.60.Lehman4μmax、KS、Topt、Tm0.421±0.011d-1、0.055±0.009μmolP/L、21.22±0.98℃、8.12±2.21℃.0~50℃21.22±0.98℃.、Anabaenaflos-aquae4632.2.3SteeleSteele4、PO3-4、PO3-4.3.SteeleRSS=0.103R2=0.448.3μmax、KS、Ik0.461±0.010d-1、0.051±0.009μmolP/L、2650.93±88.19lx.2650.93±88.19lx.2LehmanFig.2NonlinearfittingonLehmanmodelunderdifferenttemperatures3SteeleFig.3NonlinearfittingonSteelemodelunderdifferentlightintensities2.3MonodLehmanSteele.Monod、LehmanSteele、μmaxKS.μmaxKSμmaxKS19.μmaxKS.MonodμmaxKS15~30℃20℃0.447d-1、0.081μmolP/L1000~5000lxμmaxKS3000lx.Lehmanμmax、KS、Topt0.421±0.011d-1、0.055±0.009μmolP/L、21.22±0.98℃220~25℃.Foy1425℃.Steele3μmax、KS、Ik0.461±0.010d-1、0.051±0.009μmolP/L、2650.93±88.19lxLehman2650.93±88.19lxμmax3Litchman12deNobel20.μmaxμmax.8.Oliver21.33μmaxKSLehmanSteeleMonodLehmanSteele464J.LakeSci.2015273Monod.LehmanSteeleμmaxPO3-4-P<1.0μmol/LμmaxPO3-4-Pμmax.22.23.24-25.3μmaxKS.μmaxKS.MonodμmaxKS20℃0.447d-10.081μmolP/LμmaxKS3000lx.LehmanSteele、.LehmanμmaxKS0.421±0.011d-1、0.055±0.009μmolP/LTopt21.22±0.98℃Lehman0~50℃.Steeleμmax、KS0.461±0.010d-1、0.051±0.009μmolP/LIk2650.93±88.19lxSteele0~5000l
本文标题:不同磷浓度下光强温度对水华鱼腥藻Ana省略aenaflosaquae
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