不同溶解氧间歇曝气对亚硝酸盐氧化菌的影响包鹏

2016,36(9)2696~2702ChinaEnvironmentalScience,*,,,(,,,100124)(NOB),,SBR,NOB(NitrospiraNitrobacter)(55d)(113d).,NitrospiraNitrobacter,CandidatusNitrospiradefluviiNitrospira;,Nitrospira,NitrobacterNOB,Nitrobacterwinogradskyi.,,NitrobacterNOB.NitrospiraNitrobacterX703A10006923(2016)09269607Effectofdissolveoxygenonthemicrobialcommunityofthenitrite-oxidizingbacteriainanintermittentaerationreactor.BAOPeng,WANGShu-ying*,MABin,ZHANGQiong,PENGYong-zhen(KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentRecoveryEngineering,EngineeringResearchCenterofBeijing,BeijingUniversityofTechnology,Beijing100124,China).ChinaEnvironmentalScience,2016,36(9)2696~2702AbstractNitrospiraandNitrobacteraretwodominanttypesofnitrite-oxidizingbacteria(NOB).Toevaluatetheeffectofdissolvedoxygen(DO)onmicrobialcommunityofNOBundertheintermittent-aerationmode,thepopulationandmicrobialcommunityofthistwoNOBtypeswereinvestigatedinanaerationreactorwithlowDOperiod(55d)andhighDOperiod(113d).ResultsshowedthepopulationofNitrospirawasmuchhigherthanthatofNitrobacterduringthelowDOperiod(0.2~0.5mg/L),andmostclonesofNitrospirawasverysimilartothecloneCandidatusNitrospiradefluvii.AfterincreasingDOtoahighlevel(1.5~2.3mg/L)andoperatingfor113d,Nitrobacterturnedtothedominantgroup.MostclonesofNitrobacterweredistributedtothebranchcontaininNitrobacterwinogradskyi.Furthermore,thenitriteaccumulationoccurredinthisreactorduringtheshiftofpopulationbetweenNitrospiraandNitrobacterafterincreasingDO,anditgraduallydisappearedwhenNitrobacterbecamethedominantNOBgroup.Keywordsdissolvedoxygennitrite-oxidizingbacteriaNitrospiraNitrobacter(NOB)[14],.,DOpHNOB.,NOB.,[58].,:Li[6];Yang[5].,(pH),[57].,NOB20160225(51578014)*,,[e-mail]92697.,NitrospiraNitrobacterNOB[23].NitrospiraNitrospirae.NitrobacterαProteobcateria.NOB,[2].,SBR,DONOB(NitrospiraNitrobacter),,.11.1SBR12L,10L,50%,30d.(251),pH7.1~7.8,100r/min.5,10min,DO(1.3),30min,10min,10min.1.2,.,,.:pH:7.2~7.8;NH4+N:50.2~80.4mg/L;NO2N:0.12~5.43mg/L;NO3N:0.04~1.24mg/L,COD:40~55mg/L,COD.DOSBR,SBR.1.3165d,DO2:IDO(0~55d),DO0.2~0.5mg/L.DO,DO0.75mg/L;IIDO(55~165d),DO1.5~2.3mg/L.DO,DO3mg/L.1.4,pHDOWTWMulti340i(WTW,);NH4+N,NO2NNO3NQuickchem®8500(Hach,);FastDNASpinKitforSoil(QBIOgenInc.,Carlsba,CA,)DNADNA.1Table1Primerslistandannealingtemperature(5’3’)T()NitrobacterFGPS872f/FGPS1269r[10]CTAAAACTCAAAGGAATTGA/TTTTTTGAGATTTGCTAG56NitrospiraNTSPAf/NTSPAr[9]CGCAACCCCTGCTTTCAGT/CGTTATCCTGGGCAGTCCTT55EUB338f/Ntspa0685M[11]ACTCCTACGGGAGGCAGC/CGGGAATTCCGCGCTC54PCRSYBRGreen,NitrobacterNitrospira1.PCR20μL:10μLSYBR®PremixExTaq™(Takara,Dalian,China)0.4μLROXReferenceDye50,0.2μL2μLDNA.:963min;35(9530s,1,72,30s).IIINitrobacterNitrospiraPCR,1.pGEMTEasy(Progmega,Madison,WI,USA),BMJM109,LB.,100PCR,1.HhaI269836,1.NCBIBLAST,MEG4.1.1.5.:,52.5L,MLSS2000mg/L.NH4+N40mg/LNO2N20mg/L,24,pH7.2.5DO0.30.51.01.52.0mg/L.10min,6.NH4+NNO2N:NO3N,.22.1NitrospiraNitrobacterNitrobacterNitrospiraNOB,NOBNOB[1216].,PCRNitrospiraNitrobacter.1,NitrospiraNitrobacter,.(DO:0.2~0.5mg/L),Nitrospira1.62106copies/mL3.52106copies/mL,Nitrobacter,1.41105~1.96105copies/mL.NitrospiraNitrobacter,NOB.Nitrospira,NitrospiraNitrobacter[17].(DO:1.5~2.3mg/L),Nitrospira3.52106copies/mL7.69105copies/mL.,Nitrobacter7.39106copies/mL,NitrospiraNOB.,NitrobacteNitrospira,,[17].,Nitrobacter.408012016054321NitrospiraNitrbacter(106copies/mL)DODONitrobacterNitrospira(d)01NitrospiraNitrobacterFig.1NumberofNitrospiraandNitrobacterinthereactor,Liu[15]NOBNitrospira,Nitrospira;Zhang[13]Nitrobacter.,NOB.2.2NitrospiraNitrobacter.Nitrospira16s2.,100Nitrospira4(DL1DL2DL3DL4).,DL1DL2DL3CandidatusNitrospiradefluvii,94%.2.1NitrospiraNOB,92699NOBCandidatusNitrospiradefluvii.CandidatusNitrospiradefluviiNitrospira,,Park[16]NOBCandidatusNitrospiradefluvii.2NitrospiraFig.2PhylogenetictreesconstructedwiththeneighborjoiningalgorithmofNitrospirasequencesDL,DH3NitrobacterFig.3PhylogenetictreesconstructedwiththeneighborjoiningalgorithmofNitroactersequencesDL,DHNitrospira3(DH1DH2DH3),DH1CandidatusNitrospiradefluvii,84%.270036,10%(DH2)Nitrospira.mosocoviensis,Nitrospira[16].,110d,NitrospiraCandidatusNitrospiradefluvii,Nitrospira.Nitrospira,NitrobacterDODO.3,Nitrobacter,100Nitrobacter5(DL1DL2DL3DL4DL5).DL1DL2unculturedNitrobactersp.Clone6,82%;DL3unculturedbacteriumcloneMBR447,10%;DL4DL5unculturedPlanctomycetesbacterium,8%.,Nitrobacter,4(DH1DH2DH3DH4).98%Nitrobacterwinogradskyi,2%unculturedPlanctomycetesbacterium.NitrobacterNOB(2.1),Nitrobacterwinogradskyi.,,NOBCandidatusNitrospiradefluvii;NOBCandidatusNitrospiradefluviiNitrobacterwinogradskyi.2.3NOB4.(0.2~0.5mg/L)55d.4d,.,NH4+NNO3N,NO2N,.NO2N(6.1mg/L).NO2N,35dNO2N95%,.,60d..,[6,1819],.5,:,(0.3,0.5mg/L),.NOBAOB.2.12.2,NOBNitrospiraCandidatusNitrospiradefluvii.CandidatusNitrospiradefluvii(nitriteoxidoreductase,NXR),,[20].,CandidatusNitrospiradefluvii.20406080100120140160020406080100(mg/L)(d)DODONH4+-NNO3--NNO2--NNH4+-N4Fig.4Influentandeffluentnitrogenconcentrationinthereactor,92701(1.0,1.5,2.0mg/L),CandidatusNitrospiradefluvii.,CandidatusNitrospiradefluvii,.,.NOBNitrobac