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http∥.scxuebao.cn3812201412JOURNALOFFISHERIESOFCHINAVol.38No.12Dec.20141000-0615201412-1985-11DOI10.3724/SP.J.1231.2014.494352014-08-212014-10-31201203083CARS-46-172012BAD25B012012BAD25B04E-mailxj00773@163.comBiolog*510380Biolog0、15、30、4560。averagewellcolordevelopmentAWCD168h5AWCD15、30、45d060dP<0.05AWCD15、3045d4Shannon、Pielou、McIntosh060dP<0.05、、α-D--1-、L-、N--D-、40、D-PCA1PC133.9%2PC221.1%15、3045d060d。15~45d。Q938.8S965A110%90%extracellularpolymericsubstancesEPS2。、3-4、EPS2、56。、7。8。N、P9-10。、、、11-1415-16。、、30~40d17-18。、19-2021-22http∥.scxuebao.cn38。Biologcommunity-levelphysiologicalprofilingCLPP23-24。Ctenopharyngodonidellabiolog。11.1。0.2cm240g/m220m2/m210m1m0.05m×1m。。0.667hm23.0m22500kg/hm2、450kg30%。215m。2。。1.215d50、15、30、4560d。330.05m×1m350mL。500mL。1.315、、、TN。、PRO-PLUSpHpHPSH-3CTN。Biolog-ECOBiolog-ECO。0.9%25℃Biolog-ECO150mL。Biolog-ECO25℃24h590nm192h。Tiquia2572hBiolog。1.4AWCDBiologAWCDAWCD=∑Ci-R/nCi31RnECOn31AWCD。C-R0.25。Shannon-WienerH'H'=-∑Pi×logPiPiPi=Ci-R/∑Ci-R26。SimpsonD6891http∥.scxuebao.cn12BiologD=1-∑P2iPielouJsw。PielouJsw=H'/logSSloge。McIntoshUEuclidian。McIntoshU=∑n2槡iniiC-R。Biolog96BiologTMECOECOMicroPlateMatrixTechnologiesCorporation3316、12、5、4、223。56AWCD100%6。31。1.5MicrosoftOffice2007Canoco4.5。22.130℃pH7.635.0mg/L0.55±0.06~0.61±0.08ms/cmTN4.05±0.27~5.36±0.42mg/L1。1Tab.1Thephysical-chemicalcharacteristicsofthewaterinfishponds/dtime/℃temperature/mg/Ldissolvedoxygen/ms/cmconductivitypHTN/mg/L029.8±0.55.51±0.130.57±0.087.30±0.054.36±0.111529.0±0.85.26±0.170.60±0.157.67±0.084.72±0.253031.2±1.06.37±0.090.61±0.087.73±0.045.36±0.424530.8±0.25.06±0.250.55±0.067.77±0.104.05±0.276028.9±0.77.04±0.050.61±0.037.69±0.154.35±0.302.2AWCDAWCD27。AWCD24h24hAWCD48h144h1。AWCD3130d≈15d≈45d>60d>0d15d45d60d0d30d1.791>15d1.701>45d1.659>60d1.495>0d1.395。1AWCDFig.1AWCDvariationofadherentmicrobesduringbiofilmformation7891http∥.scxuebao.cn382.3ECO72hC-RShannon、Simpson、Pielou、McIntosh2。5。Shannon15、3045d060dP<0.05Simpson0d1530d4560dPielou0、15、3045d60dP<0.05McIntosh45d>15d>30d>60d>0d060d。Simpson15、3045d4060d15、30、45d。2.42。P<0.050、15、30、45、60d54.6%、96.6%、100%、92.2%88.5%30d>15d>45d>60d>0d、。0、15、30、45、60d13.8%、47.1%、25.4%、24.8%33.9%15d>60d>30d>45d>0d。45d>15d>0d>60d>30d。0d、、、。、、、。2Tab.2Diversityandevennessindicesforadherentmicrobesduringbiofilmformation/dtimeShannonindexSimpsonindexPielouindexMcIntoshindexrichnessindex03.009±0.058b0.986±0.001c0.979±0.008b5.609±0.211a21.67±1.53ab153.188±0.032c0.981±0.001b0.975±0.012b8.225±0.795c26.33±0.58c303.138±0.022c0.980±0.001b0.987±0.009b7.488±0.096bc24.00±0.00b453.103±0.035c0.977±0.002a0.981±0.017b8.090±0.238bc23.67±1.15b602.766±0.053a0.973±0.005a0.929±0.029a7.358±0.337b19.67±2.08aP<0.05n=3NoteThesamecolumnwithdifferentlettersmeansignificantdifferenceamongtreatmentsatP<0.05.n=326Fig.2Relativeutilizationratioinsixgroupsofcarbonsourcesbyadherentmicrobes8891http∥.scxuebao.cn12Biolog31313。31AWCD。。4%。331Fig.3PatternofutilizationbasedonAWCDofthe31carbonsubstratesbyAdherentmicrobesinbiofilm0d>4%615d1630d1345d1460d103。>4%AWCD15、4530d060dP<0.05>4%。0d0d13。0d4α-D--1->L->N--D->40>D->β--D->>L->80>>>9891http∥.scxuebao.cn38L->D-α-D-D-<2%2-<α-<i-<D-<L-<-L-AWCD<0.12-α-3。2.5PCA72hBiologCanocoforWindows4.5。41PC133.9%2PC221.1%3PC39.5%4PC47.7%24255.0%。PC10.55190.852PC20.5570.851。PC119PC27。4BiologFig.4PatternsofmicrobialcommunitiesinbiofilmasrevealedbyprincipalcomponentanalysisPCAofBiologplatedataprofiles15、30、45d060d060d34。3。15~45dAWCD60d0d。α-D--1-、L-N--D-13α-D-D-、、、。、。14.3%~33.3%28Besemer2930。Asaduzzaman3160d。30d。。。0991http∥.scxuebao.cn12Biologα-D--1-、L-、N--D-、40、D-、β--D-、、L-、80、、、L-、D-。α-D--1-、32-33。α-D--1-3453L-、L-L-N--D-、、35-36408037。α-D-D-。α-D-38α-D-AWCD<0.12-α-。2-392-2-。community-levelphysiologicalprofile。。31、、、。>39。。NO-2-NNO-3-N40-4143-4445-46。5PCA515、3045d060d3。47。15~45d。0d。15、3045d。60d。。、1991http∥.scxuebao.cn38。1QiHYWangWBZhengYetal.MechanismofbiofilmformationandanalysisofinfluencingfactorsJ.MicrobiologyChina2013404677-685...2013404677-685.2FlemmingHCWingenderJ.ThebiofilmmatrixJ.NatureReviewsMicrobiology201089623-633.3BullittEMakowskiL.StructuralpolymorphismofbacterialadhesionpiliJ.Nature19953736510164-167.4ThomasWENilssonLMForeroMetal.Shear-dependent‘stick-and-roll’adhesionoftype1fimbriatedEscherichiacoliJ.MolecularMicrobiology20045351545-1557.5HarmsenMYangLAPampSJetal.AnupdateonPseudomonasaeruginosabiofilmformationtoleranceanddispersalJ.FEMSImmunologyandMedicalMicrobiology2010593253-268.6RennerLDWeibelDB.PhysicochemicalregulationofbiofilmformationJ.MRSBulletin2011365347-355.7SongXFXueSSChenYM.Effectofnano-ecobaseontreatmentofaquaculturewastewaterJ.ChineseJournalofEnvironmentalEngineering2012672231-2236...2012672231-2236.8XuYT.MicrobialcharacteristicsandperformanceofbiologicalfilmsystemJ.ShanghaiEnvironmentalSciences199716320-23...199716320-23.9ZhangQZAchalVXuYTetal.AquaculturewastewaterqualityimprovementbywaterspinachIpomoeaaquaticaForsskalfloatingbedandecologicalbenefitassessmentinecologicalagriculturedistrictJ.AquaculturalEngineering201460448-55.10TangJYWangYDaiYX.Effectsofco-cultu
本文标题:草鱼养殖池塘生物膜固着微生物群落碳代谢Biolog分析李志斐
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