高浓度甲醇废水厌氧处理中颗粒污泥和产甲烷细菌的耐酸性周雪飞

:0253-2468(2004)04-0633-04　　　:X703.5　　　:A周雪飞1,任南琪2　(1.,　200092;2.,　150090):UASB,,.,UASBpH,4.9～5.85.5～6.2,,pH.,,pH5.0,,.,,pH4.5.、.:;;;;UASB;Acidresistanceofmethanogenicbacteriaintwo-stageanaerobicprocesstreatinghighconcentrationmethanolwastewaterZHOUXuefei1,RENNanqi2　(1.StateKeyLaboratoryofPollutionControlandResourceReuse,TongjiUniversity,Shanghai　200092;2.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin　150090)Abstract:Two-stageanaerobicsystemwasemployedtotreathighconcentrationofmethanolwastewater.TheresultsshowedthepHchangedfrom4.9to5.8inthefirstUASBand5.5to6.2inthesecondreactor.Apparently,thesewerenotheadvisablepHlevelsthatcommonmethanogenicbacteriacouldaccept.Themethanogenicbacteriaofthesystem,viz.Methanosarcinabarkeri,hadsomeacidresistanceandcouldstilldegrademethanolatpH5.0.Iftrainedfurther,theiracidresistancewouldbeimproved.GranularsludgeofthesystemcouldprotectthemethanogenicbacteriawithinitsbodyagainsttheimpactofacidenvironmentandmakethemdegrademethanolatpH4.5.Theperformanceofgranularsludgewasattributedtoitsstructure,bacteriaspeciesandthedistributingofbacteriuminsidethegranule.Keywords:methanolwastewater;methanogenicbacteria;acidresistance;granularsludge;UASB;batchcultureexperiment:2003-08-06;:2003-11-14:(99099):(1971—),,(),,,[1,2].::4CH3OH※3CH4+CO2+2H2O　　　　ΔG′o=-312kJ·mol-1(1)　　:4CH3OH+2CO2※3CH3COOH+2H2O　ΔG′o=-2.9kJ·mol-1(2)CH3COOH+H2O※CH4+CO2+H2O　ΔG′o=-31.0kJ·mol-1(3)　　,,,.Lettinga[3],,24420047　　　　　ACTASCIENTIAECIRCUMSTANTIAEVol.24,No.4July,2004DOI:10.13671/j.hjkxxb.2004.04.012COD17.5kg·(m3·d)-1,;,pH5.5～6.0,,[4].,,.,UASB,pH,.1　1.1　UASB,1UASB(U1)10L,4.1L;2UASB(U2)18.4L,12.5L,(35+1)℃,1.1.　2.U1　3.　4.U25.　6.　7.1　Fig.1　schematicdiagramoftwo-stageUASBprocesses　　UASBUASB,VSSSS0.42.N、P,COD∶N∶P=(200～300)∶5∶1,pH7.0.,COD8000～20000mg·L-1,12～18h.UASB20g·L-150g·L-1.1.2　2UASB,,2.10150mLAB,2　Fig.2　Setupofbatchcultureexperiment,150mL,1～5mm,A;B3～4mm,300r·min-115min,,0.5mm,,.,6000mg·L-1.pH4.0,4.5,5.0,5.5,6.05,pH.(35±1)℃,10min,.1.3　(SX-40)(H-7000)、.634　　　　　　　　　　242　2.1　3.,COD26.8kg·(m3·d)-1,,90%;,COD.UASBpH4,pH7.0,UASBpH,90%.,pH,.3　Fig.3　Theperformanceofanaerobicsystem4　pHFig.4　Thediagramofsystem'spHatvariousphase2.2　5　pH(A;B,,SS49.22g·L-1,VSSSS0.59)Fig.5　EffectofpHonmethanogenicbacteria6354:,pH6.5～7.5,pH6.,U1U2pH55.5,.,().5a,pH5.5～6.0,;pH,.pH(5b5c),,,.pH4.5,,3h;pH4.0,1.5h,.6　UASB(×7000)Fig.6　MorphologyforbacteriaongranulesludgeintheUASB(×7000)5d,pH,,pH4.5,COD26%16%,.　　.,、、、,,6.,,,,.3　　　UASB,COD26.8kg(m3·d)-1,,UASBpH,4.9～5.85.5～6.2,pH.(),pH5.5～6.0,pH5.0.,pH4.5,pH5.5,,.:[1]　.[M].:,1998:453—454,503—507[2]　WeijmaJ,StamsAJM.Mehtanolconversioninhigh-rateanaerobicreactors[J].WaterScienceTechnology,2001,44(8):7—14[3]　LettingaG,VanDerGeestATH,HobmaS,etal.AnaerobicTreatmentofMethanolicWastes[J].WaterResearch,1979,13:725—737[4]　,,　.[M].:,1993:231—238636　　　　　　　　　　24