高效净化水产养殖水域紫色非硫光合细菌的分离和筛选

收稿日期:1998-04-13郑耀通,男,1965年出生,讲师,理学硕士.研究方向:环境生物技术.*福建省教委资助项目(K96024).高效净化水产养殖水域紫色非硫光合细菌的分离和筛选*(福建农业大学土地与环境学系,福州350002),.,,,13.;;;S94;Q939SeparateandscreenpurplenonsulfurphotosyntheticbacteriausedtoefficientlypurifyaquiculturewaterqualityZhengYaotongHuKaihuiGaoShufangWangYuanzhen(DepartmentofLandandEnvironmentalScience,FAU,Fuzhou350002)AbstractThemajorobstaclesinfluencethelivabilityandproductionofhydrophilicbioticsarethewaterqualitydepravationandthetoxicityofammonia.Todepuratewaterqualityandremoveammoniaithasbecomethenodesandhotspotinaquaticsbreed.Usingdifferentorganismsastheonlycarbonsourcetodomesticateandscreendirectionallythepurplenonsulfurphotosyntheticbacteriawhichwereusedtodepurateaquiculturewaterarea.Bydeterminingthegrowthrate,biologicalactivity,abilityofutilizationcarbonsourceandremovingoforganisms,nationalizationammonia,increasingdissolveoxygenofthepurplenonsulfurphotosyntheticbacteriaindecontaminationaquiculturewaterarea,and13perfectstrainsweregained.KeywordsAquiculture;purifywaterquality;photosyntheticbacteria;screen.,,,,,..(PurplenonsulfurphotosyntheticbacteriaPSB),,.(1991),,,,,,.11.1PSB51~1.5m0.5m,.1.2/gL-1:CH3COONa3,1,CH3CH2COONa0.6,NaCl1,NH4Cl1.5,福建农业大学学报27(3):257~260,1998JournalofFujianAgriculturalUniversityMgCl27H2O0.2,KH2PO40.5,K2HPO40.6,CaCl22H2O0.05,0.1;pH6.7-7.0,1105Pa20min,.:.1.3181802530!,2000lx.1.4(1994),(TTC),(TF),TF.485nmOD,.OD722660nm(1995).NH3.CODDO(1997).22.12~3,3~4d,,2~3,1gL-1(N),1gL-1(P),1gL-1(L),1gL-1(G),0.5gL-1(B),1gL-1(S),6d,2,.,.,,YP,,,1314,2,11,210.2.2,,72h,(1).1,G2BG4AG4BG5BG5CG5E,.L2BL4BL4CL8FL8EB1BP2AP2CP2DP3BP9EN1AS2DS6AS11B.,,.S6FS11D,,,.3.33.3.1不同菌株利用生长培养基基质生长速率试验250mL,,660nm,(2).2,G2BG4BG5CG5BG5E,G4A,N1AL2BL8E343第3期郑耀通等:高效净化水产养殖水域紫色非硫光合细菌的分离和筛选P2CP3BP9ES2DS6AS11B.1Table1Thegrowthrateandbiologicalactivityofseparatedstrains(OD)(OD)(OD)(OD)G1A0.6240.557P1A1.1430.954G2B*1.4720.805P2A*1.4160.902G2E1.1070.768P2C*1.2040.968G3B0.7240.671P2H1.1600.861G3C0.8050.740P2D*1.3100.776G4A*1.4600.806P2F1.1280.831G4B*1.3100.995P3B*1.1690.991G4F0.9460.989P9A1.2240.901G5B*1.5120.995P9C1.2020.802G5C*1.3400.987P9E*1.3630.954G5H1.1350.956P9J0.6440.480G5E*1.1380.997B1B*1.2410.975G5J1.1720.974B2A1.2580.732L1A1.0420.744S1B1.1640.585L2B*1.3540.804S1C1.0600.804L2F0.8320.719S2D*1.0240.892L4B*1.1290.790S2E0.6970.712L4C*1.1720.821S6A*1.3520.933L4G0.6470.624S6D0.6280.512L4J0.9630.753S6F1.3630.953L8A0.8830.824S11B*1.1890.885L8G1.0400.840S11D1.0990.942L8L0.8020.574S11E0.8750.645L8E*1.3670.852N1A*1.4290.934L8F*1.0730.851N2B0.6630.512L11B0.8190.596L11D0.6740.5362Table2Growthrateoftheseparatedstrainsutilizingsubstrates12h24h36h48h60h72h96hG2B*0.3390.6380.9561.1501.2691.4271.123G4A0.0220.0610.0900.3120.8130.9141.230G4B*0.2980.5890.7460.9081.0671.1481.023G5B0.2240.3340.4960.8380.9471.0751.064G5C*0.3250.6040.9031.0601.1781.3341.067G5E*0.2310.3500.5420.8421.0541.1841.133N1A*0.2580.4960.7421.0331.0401.2651.110L2B*0.2370.5750.7180.9261.0381.1451.022L4B0.1890.2360.3540.5380.6660.7490.695L4C0.0900.1780.2610.5520.9901.1201.119L8E*0.2840.4940.7361.0381.0741.2101.006L8F0.2720.4570.6860.9041.0351.1411.102B1B0.2400.3760.5630.7600.6320.7670.516P2A0.2650.4670.6590.8910.9921.2100.915P2C*0.3130.5710.8531.1011.1601.2901.263P2D0.2340.3670.5420.7400.7870.8850.678P3B*0.2670.4800.6660.8950.9901.0800.941P9E*0.2530.4360.6080.8231.0031.1001.108S2D*0.3080.5670.8321.0401.1031.2100.987S6A*0.3350.6370.9381.1071.1561.3011.353S11B*0.2970.5420.7880.9801.0061.1300.983344福建农业大学学报1998年第27卷3.3.2分离菌株对不同碳源和硫代硫酸钠电子受体的利用情况,1gL-1,72h(3).3(660nmOD)Table3TheabilityofstrainsutilizingorganismsascarbonsourceandhyposulphiteaselectronreceptorG2B1.2190.2610.3050.5230.926-0.256G4B1.2940.1900.1640.533-1)1.3250.295G5C1.3450.6580.2500.2770.956-0.198L2B1.1690.1950.2510.539-0.3410.282L8E1.3170.2000.1980.443-0.8160.254P2C1.3480.5920.3930.6250.7420.316-P3B1.3150.2020.4530.5250.7691.5000.303P9E1.1320.5820.4460.5470.603-0.149N1A1.2850.7810.9100.595-0.283-S2D0.8900.3910.2010.3830.5460.1460.275S6A1.1370.3220.3180.479--0.304S11B1.3231.0160.2480.571-1.4330.1761)-.,(1995,1991).3,,,.3.4,250mL,0.01,4Table4Theresultsofscreenstrainspurifyingaquiculturewaterquality(OD)(OD)DO/mgL-1NH3N/mgL-1COD/mgL-1/%/%G2B*0.0930.1035.380.5589.3227.265.3G4B*0.0780.0924.561.1078.6440.148.9G5B*0.0630.1134.980.8084.4646.341.1G5C*0.0980.1535.670.5190.0944.043.9G5E*0.0720.1314.761.1577.6753.631.7N1A*0.0850.1394.122.0560.1952.433.2L2B0.0600.0945.022.6049.5150.136.2L8E*0.1040.1324.691.4571.8448.338.5L8F0.0860.0824.762.1558.2552.632.9P2A0.0730.1064.353.0540.7740.148.9P2C*0.1660.1685.081.6068.9338.251.3P3B*0.0920.1484.121.5569.9054.630.4P9E*0.0760.1254.071.1577.6739.250.1S2D*0.1020.1094.281.5070.8740.848.1S6A*0.1050.1384.761.7066.9932.858.2S11B*0.0610.0984.011.0579.6143.244.90.003.785.1578.5345第3期郑耀通等:高效净化水产养殖水域紫色非硫光合细菌的分离和筛选72hNH3NCOD.,G2BG4BG5BG5CG5EN1AL8EP2CP3BP9ES2DS6AS11B,NH3N.,G5C72h90%.COD,.4,CO2.,,,(),13.,13Rhodobacter,RhodopseudomsnasRhodocyclus,.,.,.,.,GS()GOGAT(),+NH+4+ATP∀GS#+ADP+Pi+H+,+-+NAD(P)H+H+∀GOGAT#2+NAD(P)+(1991).,,.,.,..GSGOGATNH+4,..O2,VB12,,,,,..().:,40,,.1994.TTC.,21(1):59,