固定化啤酒酵母废菌体吸附Cu2的研究武运

新疆农业大学学报　2007,30(4):102～105JournalofXinjiangAgriculturalUniversity:1007-8614(2007)04-0102-04Cu2+　1,1,2,1(1.,　830052;2.,　830052)　:　,Cu2+。pH、、、Cu2+。Cu2+,。,,Cu2+。Cu2+,2h。pHCu2+,pH6.0。25～45℃,。。:　;;;Cu2+:　X703.1　　　　　　:A　StudyonBiosorptionofCu2+bytheImmobilizedSaccharomycesCerevisiaeWasteBiomassWUYun1,YANGHai-yan1,ZHUJian-wen2,WANGZu-lin1(1.CollegeofFoodSciencesandTechnology,XinjiangAgriculturalUniversity,Urumqi,830052;2.CollegeEnvironmentalSciencesandResources,XinjiangAgriculturalUniversity,Urumqi,830052)Abstract:　Inthisstudy,saccharomycescerevisiaewastebiomassculturedinlabwasselectedasthebiolog-icalabsorbentandcopperastheheavymetaltobeadsorbed.ThebiosorptionofsaccharomycescerevisiaetoCu2+insinglemetalsystemwasstudiedinthedifferentconditions,suchaspHvalue,theabsorbeddose,theabsorptiontime,theabsorptiontemperatureandsoon.InordertoincreaseabsorptiveCu2+ca-pacityofsaccharomycescerevisiae,somepretreatmentsofsaccharomycescerevisiaeweremadeandimmobi-lized.TheresultsindicatedthatthemaximumabsorptiveCu2+capacityofsaccharomycescerevisiaewithHCHOwaspretreatedandgreatlyincreased.Thebiosorptionwasarapidprocess,andtheequilibriumwasachievedwithin2hours.ThepHvaluecangreatlyaffectthebiosorptionperformanceforCu2+,themaxi-mumbiosorptioncapacityofunitbiomassreachedpH6.Thetemperatureintherangeof25～45℃affect-edthebiosorptioninasmallextent.Thedensityofthebiomassaffectedthebiosorption.Keywords:　immobilize;biosorption;saccharomycescerevisiae;Cu2+　　,,、、,、,、、[1-3],[4,5]。。-[6,7]。、,,,,、,。Cu2+,,Cu2+,pH、、:2007-03-13　4　,:Cu2+。1　1.1　:、、、、(BCO)、、,。:、、、、、;、、。1.2　722();();TGL-168();pHs-2c();101A-2();SHZ-82();SHZ-DⅢ()。1.3　。1.3.1　菌体的制备(10000r/min,15min),,,,,,80℃,。1.3.2　菌体的处理:2.5g100mL,50mL,3.0mLHCl3h,,,80℃2h,,60,[8]。:2.5g17mL360g/L33mL0.1mol/L,,1h,,50mL,100mL0.2mol/L,50mL,80℃2h,,60,[9]。:2.5g50mL0.5mol,1h,,,80℃2h,,60,。1.3.3　菌体的固定-:2g1g,1.2g100mL,4%CaCl2,24h,[10-13]。:3g,。(PVA)-:2g1g,。--:、1g,。1.4　1000mg/L,。100mL,,,pH,,200r/min,1000r/min15min(),Cu2+。1.5　Cu2+Cu2+50mL,50%2mL,2,,2,4mL,0.05%BCO10mL,4min50mL,。1.6　q=(ρ0-ρe)V/m:q(mg);ρ0(mg/L);ρe(mg/L);V(L);m(g)。2　2.1　,。、、(1)。1,,。,,。。,,。,。103　　　　　　　2007　2.2　ρ0=50～250mg/L,m=0.1g(),pH=6.0,t=30min,(2)。2,-,。1　2　Fig.1　Effectofbiomasspretreatmentonabsorptiveca-pacityFig.2　Comparisonofbiomasscapacityfordifferentim-mobilizedsorbents2.3　pHρ0=40mg/L,m=1g,t=30min,pH=3.0～7.0,pH(3)。3,pH6.0,Cu2+。pH3.06.0,Cu2+pH。pH6,Cu2+pH。:pH,。pH,H+H3O+,,,;pH,OH-,,。2.4　ρ0=100mg/L,m=0.1g,pH=6.0,(4)。3　pH4　Fig.3　EffectofpHonabsorptivecapacityFig.4　Effectofabsorptiontimeonabsortivecapacity　　,Cu2+。10min,2h(4)。,Cu2+。2.5　ρ0=40mg/L,m=0.5～1.3g,pH=6.0,t=12h,(5)。Cu2+,,Cu2+(5)。Cu2+。,,,,,,。2.6　ρ0=40mg/L,m=1.0g,pH=6.0,T=25～45℃,t=2h,(6)。104　4　,:Cu2+5　6　Fig.5EffectofbiomassonabsorptivecapacityandabsorptionrateFig.6　Effectoftemperatureonabsorptiveeapacity　　Cu2+,30℃(6)。3　Cu2+90～120min,。25～45℃,Cu2+,。,,Cu2+,。pHCu2+,pH=6。Cu2+,,Cu2+,Cu2+,,。,Cu2+,。4　,,,,,。,,。,,,。,,,。:[1]　,.[J].,1998,14(2):180-187.[2]　,,.[J].,2000,10(1):33-36.[3]　,,.[J].,2000,27(6):449-452.[4]　,,.[J].,2005,21(2):122-126.[5]　ChenHong,PanShan-shan.Bioremediationpotentialofspirulina:toxicityandbiosorptionstudiesoflead[J].ZhejiangUnivSCIB,2005,6B(3):171-174.[6]　,,.[J].,2002,15(2):26-29.[7]　,,,.[J].,2000,26(4):240-244.[8]　,,,.[J].,2000,32(3):72-75.[9]　,,,.[J].,2005,24(1):34-37.[10]　,.[J].,1996,11(2):45-50.[11]　,,.[J].,1990,12(1):72-76.[12]　,,.[J].,2000,18(3):49-51.[13]　,,.Pd2+[J].,2003,30(6):29-34.105