荷花不同部位总黄酮分布及荷叶黄酮提取工艺研究郭明

:,,　　Tel:[phone]　　E-mail:[e-mail]郭明1,2,杨群超2,朱灵芝2,吕圭源1(1.,310053;2.,311300):　测定荷花不同部位总黄酮含量分布并探讨荷叶黄酮最佳提取工艺条件。　采用索氏提取法对荷花五个不同部位(花瓣、荷叶、叶梗、莲蓬壳、雄蕊)的总黄酮进行提取,利用硝酸铝显色法测定提取总黄酮含量;采用超声波辅助回流法提取荷叶黄酮并通过正交实验设计进行工艺条件优化。　荷花不同部位总黄酮含量分布:莲蓬壳荷叶雄蕊花瓣叶梗;超声波辅助回流法提取荷叶黄酮时用30倍量60%乙醇,70℃超声回流提取90min,其中超声时间为20min为佳。　荷花不同部位总黄酮含量有一定差异;在超声波辅助回流法提取荷叶黄酮实验处理下,不同提取工艺条件对荷叶黄酮提取效果产生一定的影响。:荷花;黄酮;提取工艺;分光光度法:R284.2　　　:A　　　:1007-7693(2009)03-0211-05TotalFlavonoidDistributionintheDifferentPartsofLotusandStudyontheExtractionTechnologyofFlavonoidinLotusLeafGUOMing1,2,YANGQunchao2,ZHULingzhi2,LVGuiyuan1(1.ZhejiangChineseMedicalUniversity,Hangzhou310053,China;2.DepartmentofChemistry,ZhejiangForestryUniversity,Lin′an311300,China)ABSTRACT:OBJECTIVE　Todeterminethetotalflavonoiddistributioninthedifferentpartsoflotusandexploreonthebestextractiontechnologyofflavonoidinlotusleaf.METHODS　Thetotalflavonoidsoflotuspetal,lotusleaf,lotusstalk,lotusreceptaculumlotiandlotusstamenwereextractedbySoxhlet′sapparatusmethod,theNaNO2-Al(NO3)3-NaOHsolutionwasadoptedasdevelopmentsystemandrutinasthestandardsample,andthetotalflavonoidsfromthefivepartsoflotuswerethendeterminedthroughultravioletspectrophotometerandtheirdistributionwascalculated.Theultrasonic-assistantrefluxextractionmethodwasalsousedtoextracttheflavonoidsoflotusleaf,andtheoptimumextractionconditionwasexploredusingorthogonaldesign.RESULTS　Thetotalflavonoiddistributioninthedifferentpartsoflotusisasfollows:lotusreceptaculumlotilotusleaflotusstamenlotuspetallotusstalk.Theextractiontechnologyofflavonoidinlotusleafbyusingultrasonic-assistantrefluxextractionmethodwasgottotheoptimumcondition:30foldof60%alcoholsolutionproportiontothelotusleafmaterials,ultrasonic-assistantrefluxextraction90minunderthetemperature70℃,andultrasonictime20min.CONCLUSION　Thecontentoftotalflavonoidvariesinthedifferentpartsoflotus;theextractionefficiencyofflavonoidoflotusleafhasbeeninfluencedtosomeextentunderdifferentexperimentalconditionusingultrasonic-assistantrefluxextraction.KEYWORDS:lotus;flavonoids;extractiontechnology;ultravioletspectrophotometricmethod　　,。[1]。[2-4],,,“”[5]。,、[6]。Elegami[7]NympholideA,NympholideBMyricetin-3′-O-(6″-p-coumaroyl)-glucoside3,[8](Hyperons)、(Isoquercetin)、(Astragalin)、(Quercetin)4。、、、、,[9-13]。,[14-17],。,,,,。,,,,,。·211·20093263　　　　　　　　　　　　　　　ChinJMAP,2009March,Vol.26No.3DOI:10.13748/j.cnki.issn1007-7693.2009.03.0091　(≥95%,,);(AR);(AR);(AR);(AR);(AR)。(HH-4,);;(AEG-220G,Shimadzu);-(UV7500,Shimadzu);KQ-250DB();SENCOR201();DHG-9123A()。2　2.1　[18]、、、、(8)。[(50±1)℃],,。2.2　、、、、。10mL10min,,3mL,5min,。,3mL,5min,,[19]。2.3　2.3.1　　(、、、、)5.0g,、,60%200mL3h,3,250mL。2.3.2　　NaNO2-Al(NO3)3-NaOH,UV7500-。[20],,Al(NO3)3,,,。2.3.3　　60%0.205mg·mL-1,5mL50mL,60%。5mL,5%NaNO22.5mL,6min,2.5mL10%Al(NO3)3,6min,10mL5%NaOH,15min,270～650nm,。2.3.4　　0.205mg·mL-12.0,4.0,6.0,8.0,10.0,12.0mL650mL,60%,NaNO2-Al(NO3)3-NaOH(510nm)A,A、(mg·L-1)。2.3.5　　10mL50mL,NaNO2-Al(NO3)3-NaOH510nm,。2.4　2.4.1　　2.0g5,、、、、,,(55℃,100r·min-1),80mL,20mL,100mL,5mL,60%50mL。“2.3.3”,510nm。2.4.2　　2.0g,、、3,3,100mL,510nm,L9(33),1、2。1　正交因子水平表L9(33)Tab1　FactorsandlevelsoforthogonalexperimentA/%B/(W∶V)C/min1601∶10202701∶20253801∶30302　L9(33)正交表Tab2　L9(33)orthogonaltableABC111121223133421252236231731383219332·212·ChinJMAP,2009March,Vol.26No.3　　　　　　　　　　　　　　　200932633　3.1　、、、、5,3。3　荷花各部位黄酮的定性显色结果Tab3　Theresultsofcolorreactionaboutfivepartsoflotus　　3′、4′-,。-,。,。3,[19,21]。3.2　,510nm,A,,,510nmA。1,y-(mg·L-1);x-A(510nm)。8.2～49.2mg·L-1。1　黄酮(芦丁)含量的实验标准曲线Fig1　Thestandardcurveofflavonoid(Rutin)content3.3　2。,,、、,。2　索氏法提取荷花五部位材料的黄酮含量棒状分布图Fig2　Thedistributionofflavonoidcontentsinfivepartsoflotus3.4　3.4.1　　60℃30min,。,。60%,,60%。3.4.2　　60℃,60%30min,。,,1∶30,,,,1∶30。3.4.3　　,1∶3060%30min,。,70℃,70℃。3.4.4　　70℃60%,,30min。,,,90min,90min。3.4.5　　70℃60%90min,·213·20093263　　　　　　　　　　　　　　　ChinJMAP,2009March,Vol.26No.3,。,,。20min,20min。3.5　“2.4”,、、,4,5。4,A1B3C1,3060%,70℃90min,20min。5,。,7.63‰。4　,,。,(),,4　正交试验结果分析Tab4　ResultsoforthogonalexperimentsABC/‰11115.03921226.44731337.55842124.91652235.18762316.69473134.54583216.44793324.644K119.04414.50018.180K216.79718.08116.007K315.63618.89617.290k16.3484.8336.060k25.5996.0275.336k35.2126.2995.763RK3.4084.3962.173Rk1.1361.4660.7245　方差分析Tab5　Analysisofvariance(s)(f)(s/f)F(A)2.00221.0010.697F0.99(2,2)=99.0-(B)3.64721.8241.271F0.95(2,2)=19.0-(C)0.79620.3980.278F0.90(2,2)=9.00-(e)2.87221.436,,。,、,,7.63‰,,。。REFERENCES[1]XUDS.ChineseLotus()[M].WuHan:WuhanInstituteofBotany,ChineseAcademyofSciences,1987:46-53.[2]DINGYM,YEDQ,ZHOUMQ,etal.Inhibitoryeffectsofflavonefromthelotusongallstoneformationanditsmechanisminrabbits[J].MedJWuhanUniv(),2007,28(5):573-575,591.[3]JINSR,YAOLF,LIH,etal.Studyontheextractionofflavonoidsfromlotusleafanditsantionxygenation[J].AnhuiAgriSciBull(),2006,12(8):40-41.[4]CHENHG,ZENGQX.Studyontheextractingtechnologyoffunctionalcomponentsfromlotusleafandtheeffectonscavengingoxygenfreeradicals[J].FoodFermentInd(),2000,27(10):34-38.[5]FANJS,HANXG.FoodAdditives()[M].TianJing:TianjinScienceandTechonlogyPublishingHouse,1993:10.[6]ZHONGXF.Studiesonextract