湖泊草藻来源溶解性有机质及其微生物降解的差异

34320143ActaScientiaeCircumstantiaeVol．34No．3Mar．2014No．ZＲ2012DQ003No．41301544“”No．NIGLAS2012135002SupportedbytheNaturalScienceFoundationofShandongProvinceNo．ZＲ2012DQ003theNationalNaturalScienceFoundationofChinaNo．41301544andthe“1-3-5”PlanningProgramofNanjingInstituteofGeographyandLimnologyChineseAcademyofSciencesNo．NIGLAS20121350021982—[e-mail]*[e-mail]1982—[e-mail]*[e-mail]10．13671/j．hjkxxb．2014．0120．2014．、J．343688-694YaoXZhangYLZhuGWetal．2014．DifferentdegradationmechanismofdissolvedorganicmatterderivedfromphytoplanktonandmacrophytesinLakeTaihuChinaJ．ActaScientiaeCircumstantiae343688-694、122*21．2520002．2100082013-07-102013-08-092013-08-09、DOM．、DOM．DOM．DOMDOMDOMC3、DOM．、DOM2d70%DOM．0253-2468201403-688-07X171ADifferentdegradationmechanismofdissolvedorganicmatterderivedfromphytoplanktonandmacrophytesinLakeTaihuChinaYAOXin1ZHANGYunlin2ZHUGuangwei2*QINBoqiang21．SchoolofEnvironmentandPlanningLiaochengUniversityLiaocheng2520002．StateKeyLaboratoryofLakeScienceandEnvironmentNanjingInstituteofGeographyandLimnologyChineseAcademyofSciencesNanjing210008Ｒeceived10July2013receivedinrevisedform9August2013accepted9August2013AbstractLakeTaihuhashighspatialvariabilityincludingregionsdominatedbyphytoplanktonormacrophytes．TherearemultiplesourcesofDOMinLakeTaihuincludingallochthonousriverinputandautochthonousbiologicaldegradation．WeinvestigatetheproductionofDOMfromthedecompositionofAlgaepulpandP．malaianusinwell-controlledlaboratoryexperimentsrespectively．Theprotein-likefluorescencewasthemostimportantcomponentinbothalgal-dominatedDOMandmacrophyte-dominatedDOM．Howeverthetryptophan-likefluorescencewasthemostimportantcomponentinthealgal-dominatedDOMwhilethetyrosine-likefluorescenceinthesoutheasternmacrophyte-dominatedDOM．Theseresultsweresimilarwithfieldinvestigations．Intheexperimentabout70%ofthetotalalgal-dominatedDOMwasfoundtobereadilydegradablein2dayswhiledegradationcycleofmacrophyte-dominatedDOMtooklongertime．KeywordsTaihuLakeDOMphytoplanktonmacrophytesdegradation1Introduction20082010．DOM、、、、3、Simonetal．2002Turner2002．DOMDOMDOMMakietal．2010．、、、DOMTzortziouetal．2008Zhangetal．2009．、DOMQinetal．20042005Yaoetal．2011．DOMZhangetal．2009DOM、DOM、、DOM、DOMDOM．2Materialsandmethods2．120～25℃GF/C、DOM．DOM3．10dpHMillopore0．2μmDOM．T1、T2、．2．2DOMF-7000FLSpectrophotometer5nm200～450nm5nm250～600nm1nmMilli-Q0．01mg·L－1．2．3PAＲAFACPAＲAFACDOM3．PAＲAFACALSxijk=∑Ff=1aifbjfckf+eijki=12…Ij=12…Jk=12…Kxijki=k、=jaififbjfckffj、keijkF．2．4MATLABDOMFluorPAＲAFAC．SPSS11．5、、．．p＞0．050．01＜p＜0．05p＜0．01．3Ｒesults3．1pHDOMDOMpH6pH6．891．DOMDOMpH5.48DOMpH26．1110pH6．591．tpHp＜0．01．986341pHFig．1ChangesofpHintheprocessofdecomposition3．2DOMPAＲAFACDOMPAＲAFAC4C1～C42．4Stedmonetal．2005a2005bYamashitaetal．2008YamashitaandJaffe2008．C1220nm280nm320nm．C2220nm270nm357nm．C1C2Stedmonetal．2005bYamashitaetal．2008YamashitaandJaffe2008T．C3220nm275nm304nmStedmonetal．2005bYamashitaetal．2008BC1、C20963、24Fig．2TheEEMcontoursofthefourfluorescentcomponentsidentifiedbyPAＲAFACandsplit-halfvalidationresultsforfourcomponents．C4220nm290nm395nmAMYamashitaandJaffe2008PAＲAFAC．C1、C2C3C4．3．3DOM10dDOM90%DOM3．2d70%．DOM7d37%5．37%·d－13d50%16．77%·d－1．3、DOMFig．3DynamicsofDOMdegradationofalgae-derivedandsubmergedplant-derivedDOMextracts196343．4DOM4、DOM4．DOMC1～C31C28d0．C44．46%32．56%4．4DOMFig．4CompositionofDOMfluorescenceoffourcomponentsandchangesintheircontributiontototalDOMfluorescenceintensityDOMC3C3p＜0．017dC3．C20．7dC2C37d90%42%32%C1C4．4Discussion4．1DOMDOMDOM．、DOMDOMDOMThomasetal．1995．、、YamashitaandTanoue2008Wangetal．2007．pH．、DOM4．DOMC1、C280%C320%．DOMC390%C1．、DOMDOMDOMYaoetal．2011．、DOM、DOM、DOM．、DOMDOM＜5%．DOMKarmeretal．2004Nieto-Cid2963、etal．2006．IseBayJapanDOM25%Karmeretal．2004．71dYamashitaandTanoue2004．4．2DOMDOMQuallsetal．2003Jaffeetal．2004．、DOMDOMDOMHamamachietal．2008．DOM、DOM3．2d70%10d．、Hulattetal．2009．、．Scullyetal．2004．DOMHamamachietal．2008．5Conclusions1DOM．2DOMDOMDOMC3．、DOM、DOM、DOM．3、DOM2d70%DOM．ＲeferencesHanamachiYHamaTYanaiT．2008．DecompositionprocessoforganicmatterderivedfromfreshwaterphytoplanktonJ．Limnology957-69HulattCJThomasDNBowersDGetal．2009．ExudationanddecompositionofchromophoricdissolvedorganicmatterCDOMfromsometemperatemacroalgaeJ．EstuarineCoastalandShelfScience84147-153JaffeＲBoyeraJNLuXetal．2004．Sourcecharacterizationofdissolvedorganicmatterinasubtropicalmangrove-dominatedestuarybyfluorescenceanalysisJ．MarineChemistry84195-210KramerGDHerndlGJ．2004．Photo-andbioreactivityofchromophoricdissolvedorganicmatterproducedbymarinebacterioplanktonJ．AquaticMicrobileEcology36239-246MakiKKimCYoshimizuCetal．2010．Autochthonousoriginofsemi-labiledissolvedorganiccarboninalargemononicticlakeLakeBiwacarbonstableisotopicevidenceJ．Limnology112143-153Nieto-CidMAlvarez-SalgadoXAPerezFF．2006．MicrobialandphotochemicalreactivityoffluorescentdissolvedorganicmatterinacoastalupwellingsystemJ．LimnologyOceanography511391-1400QinBQHuWPGaoGetal．2004．Dynamicsofsedimentresuspensionandtheconceptualschemaofnutrientreleaseinthela