全基因组扩增

DNA(Qnr.Cn)2010/3/819:51:27(PCR)DNAPCRDNAPCRwholegenomeamplification,WGADNAIRS-PCRinterspersedrepeatsequencePCR1LA-PCRlinkeradaptorPCR2T-PCRTagged-PCR3DOP-PCRdegenerateoligonucleotideprimedPCR4PEP-PCRprimerextensionpreamplificationPCR5DOP-PCRPEP-PCRDOP-PCRPEP-PCRDNADOP-PCR356PCRDOP-PCR34DOP-PCRPEP-PCR15PCR5037555DNADOP-PCRPEP-PCRDNACheung6DOP-PCR122DNAPEP-PCRZhang5PEP-PCR78%30PEP-PCR10007DNA8,DNADuddy93m1mm2PEP-PCR10100PCRDOP-PCRPEP-PCRDNADNA410DNA4WGAPCRDNATaqDNAVentWGA7WGA835DNA3Dietmaier8WGAPEP-PCRDOP-PCRDOP-PCRPEP-PCRWells11EBDOP-PCRPEP-PCRDOP-PCRPEP-PCRWGADNAPEP-PCR41510PEP-PCR12DOP-PCR461113DOP-PCRPEP-PCRDOP-PCRDOP-PCR()PEP-PCRalleledrop-out(lossofheterozygo-sity)6-8111314DNA15WGADNAWGAPCRWGA6-8WGA14WGAPCR11Dietmaier810SW480PEP-PCRBarret1450100Dietmaier8250Kim13DNA20ng1000WGA7WGAPCRDNATaq1617WGATaq781118Taq8DNAGC11WGAWGA19DNADOP-PCRPEP-PCR500bp6111419PEP-PCR800bp5DOP-PCR1.5kb11DOP-PCRPEP-PCRDOP-PCRDNAPEP-PCRDNAWGAWGADNADOP-PCR4201113DNA11PEP-PCR57188914WGA814WGAPCRWGAWGADNADNADNARNAcDNA(973)(G1998051207)100021100021100021