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2003.251.2.3.1/104.5.---------6.pH3.57.RNA()8.tRNA9.10.RNARNA,DNADNA()11.DNA12.trpoperon13.14.RecADNANTP15.pBSKlacZ16N-17.18.19.20.21.pH22.23.24.NADHP/O325..201.1.2.3.4.2.1mol/L1.22.33.64.83.pI8pH61.2.3.4.4.A,B,C,DABCD,1.A;2.B;3.C;4.D5.1.-2.-3.-4.6.DNADNA1.4dNTP;2.4NTP;3.4dNDP;4.47.1.102.203.504.1008.IGC1.GCG;2.GCA;3.ACG;4.ICG9.RNA2C1.RNA22.RNA2DNA3.RNA24.10.GAL4DNA1.2.3.--4.--11.NA11.tRNA5sRNA2.snRNA3.rRNA4.12.1.DNA2.3.DNA4.430bp13.1.Y;2.3.4.14.1.2.Vmax3.4.Km15.16.1.2.3.4.v817.A;2,4-18.A1.2.3.4.19.ATPATP1.2Na+,2K+;2.3Na+3K+;3.2Na+,3K+;4.3Na+2K+;20.Ca+21.1/10002.1/2003.1/504.1/10.251.2.3.Northern4.DNADNA1DNA2DNArRNA,mRNA,5.DNA6.7.8.DNARNA29.10.11.12.13.414.C15.16..10*81.20N=D,ND1.0*10^(-8),.(R=1.9872cal/(mol*K)R=8.3144J/(mol*K))2.3.RNA4.5.HIV6.7.28.9.ADP3NAD+10.2003():,!!1.dG''=-2.303RTlnK.2.(37,!!)(genome)mRNAmRNA(transcriptome)mRNAmRNA(proteome)-DNA(proteomics)1.Western2.mRNA3./--4.-LCMLCM-mRNALCM20003000/MALDI-TOF;()2003()(),!!3.RNA,:(MRNA),(TRNA),ribozyme,Genesilencing,(micRNA),rRNA--------ribozyme,RNA/,RNAediting(guideRNA),Genome,DNA.4.5.HIV+RNA,CD4,,,GENOME,.67893NAD+10:6.Pi,,,:,,.,H1PROTEINS.7.:(1)(2)().:.,---------.().(),,,X-RAY,.8.;.PROTEINFOLDINGFOLDING;FOLDINGFOLDING,..PROTEINFOLDINGFOLD,PPI,PDI;FOLDINGFOLD,PPI,PDI.9.10.:.
本文标题:中科院2003年生物化学试题
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