双体系双波长荧光分析法同时测定废水中的间苯二酚和对苯二酚

,2008,28(2):19222MetallurgicalAnalysis,2008,28(2):19222:1000-7571(2008)02-0019-043,,(,237012):,280nm,30910nm(pH210)/35817nm(pH710)32916nm(pH710)/35817nm(pH210),,11010-811010-5mol/L11010-851010-5mol/L,9513%10110%9413%9710%:;;:O657131:A:2007-02-05:(1966-),,,,,Tel:[phone],E2mail:cgxie@iim1ac1cn,,,,,:[1-2][3][4][5][6][7],[8],,,,,,,,,(pH710)(pH210),,,,1111RF5031PC();pHS23():11010-2mol/L,,;:011mol/L,91XIECheng2gen,WUWei,LIUXiao2lan.Simultaneousdeterminationofresorcinolandhydroquinoneinwastewaterbydouble2systemdouble2wavelengthfluorometry.MetallurgicalAnalysis,2008,28(2):19222112100mL,(pH210)(pH710),280nm,3001045010nm,pH210()1(30910nm)32(35817nm)pH710()2(35817nm)31(32916nm)(F1,F32,F2,F31),,F=F1-F2F=F31-F322211pH21011010-5mol/L,,3nm,,1ex=27318nmem=30819nm;ex=28910nmem=32910nm1,,1(A)(B)Fig.1Excitation(A)andemission(B)spectra1,1.11010-5mol/L(Resorcinol);2,2.110-5mol/L(Hydroquinone),(1A),280nm,212280nm,300450nm,,,,,pHpH,,(pH710)(pH210)213280nm,pH210()pH710(),2,,pH2101=30910nm,pH7102=35817nm,,pH=71031=32916nm,pH21032=35817nm2Fig.2Selectionofwavelengthpairsindifferentsystem1.(Resorcinol);2.(Hydroquinone)pH2101(30910nm)pH7102(35817nm),(F1,F2):02,,..,2008,28(2):19222F1=K1C+K1C(1)F2=K2C+K2C(2)pH2101(30916nm)pH7102(35818nm),(2),:K1C=K2C(3)(1),(2)(3):F=F1-F2=K1C-K2C=(K1-K2)C(4)FC,,,214,12h,12h215,11010-811010-5mol/LF,:F=F1-F2=-36122+4172107c(mol/L),r=019993;51010-811010-5mol/LF,:F=F31-F32=24190+3164107c(mol/L),r=0199943,(pH210)(pH710),,,,1,,,1(n=7)Table1Determinationresultsforresorcinol(RES)andhydroquinone(HYD)inwastewater(n=7)SampleNo1(mol/L)FoundRESHYDRSD(%)RESHYD(mol/L)AddedRESHYD(mol/L)RecoveredRESHYD(%)RecoveryRESHYD111102210340195017411000210000196211899961295102110345147801381110110006100001953516569513941336174511121016301686100011000610050197010110971041198511035016101862100011000119680196398149613:[1],,1LM2BP[J]1(SpectroscopyandSpectralAnalysis),2005,25(12):2070220721[2],,1[J]1(ChineseJournalofAnalyticalChemistry),1997,25(9):1024212101[3],,1[J]1(Chi2neseJournalofAnalyticalChemistry),1999,27(3):33123331[4]PennerNA,NesterenkoPN1Simultaneousdetermi2nationofdihydroxybenzenes,aminophenolsandphe2nylenediaminesinhairdyesbyhigh2performanceliquidchromatographyonhypercross2linkedpolystyrene[J]1Analyst,2000,125(7):1249212541[5],,1[J]1(ChemicalJournalofChineseUniversities),2000,21(6):87628781[6],,1[J]1(ChineseJournalofAnalyticalChemistry),2000,28(8):96829701[7],,1[J]1(ChineseJournalofAnalyticalChemistry),2003,31(3):35723591[8],1[J]1(ChineseJournalofAnaly2sisLaboratory),2001,20(6):88289112XIECheng2gen,WUWei,LIUXiao2lan.Simultaneousdeterminationofresorcinolandhydroquinoneinwastewaterbydouble2systemdouble2wavelengthfluorometry.MetallurgicalAnalysis,2008,28(2):19222Simultaneousdeterminationofresorcinolandhydroquinoneinwastewaterbydouble2systemdouble2wavelengthfluorometryXIECheng2gen3,WUWei,LIUXiao2lan(DepartmentofChemistryandLifeScience,WestAnhuiUniversity,Liu’an237012,China)Abstract:Basedonthefluorescentdifferenceofresorcinolandhydroquinoneatdifferentacidmedium,anovelmethodforsimultaneousdeterminationofresorcinolandhydroquinonebydouble2systemdoub2le2wavelengthfluorometrywasdeveloped1Theexcitationwavelengthofthereactionsystenisat280nm.ThewavelengthpairsfordeterminingresorcinolandhydroquinoneatpH2(HCl)andpH7(H2O)are30910nm(pH210)/35817nm(pH710)and32916nm(pH710)/35817nm(pH210),re2spectively.Thefluorescenceintensitydifferencesobtainedbybothwavelengthpairsarelinearwiththeconcentrationofresorcinolandhydroquinoneintherangeof11010-8-11010-5mol/Land11010-8-51010-5mol/L,respectively1Themethodhasbeenusedforthedeterminationofresor2cinolandhydroquinoneinthelaboratorywastewaterwiththerecoveriesrangingfrom9513%to10110%and9413%to9710%,respectively1Keywords:double2systemdouble2wavelengthfluorometry;resorcinol;hydroquinoneChinaNILResearchCenterforProficiencyTesting(NIL),20068(No.CNASPT0002),ISO/IEC43-1997ILACG13:2000,,,,200,626800/,48535/,,2008,33,21():7614,:100081::[phone]/6287/1165:[phone]E2mail:[e-mail]/[e-mail]22