野香苏籽油中多价不饱和脂肪酸的提取及纯化工艺

27220083JournalofFoodScienceandBiotechnologyVol.27No.2Mar.2008:167321689(2008)0220061203:2007201210.:(2001ABAMA02A005).:(19632),,,,.Email:[e-mail]1,1,2(1.,653100;2.,650091):,(L934)(+)43616%,3615%,2617%,3710%,2:1100g/mL,(+)1118,7378,40min:;;;:TS23512:AStudyonExtractionandPurificationofPUFAfromElsholtziarugulosaSeedOilMAOShao2chun1,LIZhu2ying1,LICong2(1.DepartmentofAgriculturalandForestry,YuxiAgriculturalVocationalInstitute,Yuxi653100,China;2.Col2legeofChemistryandMaterialEngineering,YunanUniversity,Kunming650091,China)Abstract:Elsholtziarugulosaseedoilwereextractedbysqueezingmethod,solventextractionmethod,moleculardistillationmethodandsupercriticalextraction.Polyunsaturatedfattyacid(PUFA)ofElsholtziarugulosaseedoilwereenrichedbyureainclusionmethod.Ureainclusionreactionparameters,suchasconcentrationofureainethanol(g/mL),theweightratiooffattyacidswithureaandethanol,timeandtemperature,wereinvestigatedbyorthogonaldesigntest(L934).Theresultshowedthatfourkindofextractionmethodoilextractionratiorespectivelywas3616%,3615%,2617%and3710%.Itsprincipalcompositionswasalpha2thelinolenicacid.Thebestextractiontechnologicalconditions:concentrationofureainethanol,fattyacid/(urea+ethanol),temperatureandtimerespectivelywas1100g/mL,1118,7378and40min.Keywords:Elsholtziarugulosa;fattyacid;extraction;purification(ElsholtziarugulosaHemsl),,12003500m[1],[2]©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[3-4],,11116YL295,;KDL5,UIC;HA221250206,;:;,,301121121110kg,95,112122kg,,,2h,,1121365012kPa1D/s370390r/min45,2h,11214I1015MPa,75II68MPa,45,5kg/h2kg,,CO21h,20min,113HP6890AGC/HP5988MSGC:SE254:59m,:0132nm,201,:230,100250,:4/minMS:250,:250,:GC,70eV,1800V,301000,SCAN114100g400mL40%2,802h,,10%HClpH23,,,,,,[5]115,,,,,,-1024h,,,10%HClpH23,,,,,,,,,(+)4,ABCD(L934)11Tab.1FactorsandlevelsoforthogonaldesigntestA/(g/mL)m()m(+)BC/minD/10150111540606520175111860667231100121080737822114422,42,3710%6110%,,2617%5615%,24,,,,,2627©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:+/,BACD:A3B2C1D3,1100g/mL(+)111840min7378,96150%,268184%,,24Tab.4ResultoforthogonaltestABCD/%1111118.352122261.933133375.524212351.795223195.826231289.167313265.978321396.509332188.77K151.9345.3768.0067.64-K278.9284.7567.5072.35-K383.7584.4879.1074.60-R331.8139.3811.606.96-5Tab.5VarianceanalysisFA1763.782.00881.8923.313F0.05(2,2)=19B3080.872.001540.4340.713F0.01(2,2)=99C258.042.00129.023.41D75.682.0037.843,4,,,,,,,,,2,2(References):[1].[M].:,1974.[2].[M].:,1977.[3]ErikBS1Thedetectionofn23fattyacidsonplasmalipidsandlipoproteinsandothercardiovascularriskfactorsinpatientswithhyperlipidemia[J].Atherscclerosis,1993,103:107.[4],.[J].,1996,14(2):77.QIULei,JIANGYuan2ying.Studyprogressonpharmacologicactionofpolyunsaturatedfattyacids[J].JournalofPhar2maceuticalPractice,1996,14(2):77-80.(inChinese)[5],,,.[J].,2005,(5):33-34.LIMing,ZHANGLian2fu,LIJi2xin.Purificationoflinoleicacidfromsaffloweroilwithureaadductionfractionation[J].ScienceandTechnologyofCereals,OilsandFoods,2005,(5):33-34.(inChinese)(:)362:©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.