大肠杆菌外膜蛋白OmpF结构研究进展-赵志平

25120122JournalofSichuanUniversityofScience＆EngineeringNaturalScienceEditionVol.25No.1[e-mail]1673-1549201201-0006-04OmpF11211111．6430002．400044OmpF600Da。OmpF、。OmpF。OmpFLoop3。OmpFLoop3Q81A、、1、2-4。5。OmpC、OmpFPhoE6-7。OmpF600Da8。OmpF。1OmpFOmpF。1991Weiss9Rhodobactercapsulatus16β-anti-parallelβ-barrel10。1992CowanOmpFRb．sphaeroidesOmpF16β-8longloop8shortturn。8T1、T2、T3、T4、T5、T6、T7T8β-。8L1、L2、L3、L4、L5、L6、L7L811。L1、L4～L86OmpF5nm121PDB3HWB34012。16β-7～16。L2OmpF13。1K－12MG1655OmpFOmpF31．59。ab。PDBOmpF。1995Cowan14OmpF23．2。。OmpFOmpF。2010Kefala155mMFC12fos-choline－12OmpF。3.8a=136.7b=210.5c=137β=100.5°。4.4a=215.5b=215.5c=137.5γ=120°。FC12OmpF42。2FC－12OmpFOmpF16β-23－28aa、30－45aa、61－73aa、76－88aa、105－113aa、116－122aa、157－163aa、172－181aa、194－204aa、206－217aa、233－244aa、246－257aa、275－287aa、290－303aa、328－337aaUniProtKB/Swiss－ProtP02931。2OmpFLoop3L1－L88L3、、、、16-17。L35β-18。L3103－134Asp107、Asp113、Asp121、Asp126Asp127、11716。1991JeanteurL3PEFGG19。1998Bainbridge175OmpFNA55OmpFL3。2001Phale20OmpFL3D113E117OmpF、D113E117N113Q117OmpF2。2003Bredin8OmpFL3OmpFOmpFAN、OmpFL3D113D121。2007BegicWorobecL3G124OmpF。2008Yamashita21OmpF1.6OmpFL3Asp113Glu117。L3OmpF。Mg2+Mg2+L3Asp113Glu117Mg2+L3。L3。3OmpFOmpC、OmpFPhoE7LPSLPS22-25。OmpFOmpCPhoE60%3DNAMAN。PEFGG3。GenebankBLASTOmpF。PCROmpF7251OmpF。3K12MG1655OmpC、OmpFPhoE4、。。1CascalesEBuchananSKDucheDetal．Colicinbiolo-gyJ．MicrobiolMolBiolRev2007711158-229．2NikaidoHVaaraM．Molecularbasisofbacterialoutermem-branepermeabilityJ．MicrobiolRev19854911-32．3BekhitAFukamachiTSaitoHetal．TheroleofOmpCandOmpFinacidicresistanceinEscherichiacoliJ．BiolPharmBull2011343330-334．4Queralt-MartinMGarcia-GimenezEMafeSetal．DivalentcationsreducethepHsensitivityofOmpFchannelinducingthepKashiftofkeyacidicresiduesJ．PhysChemChemPhys2010132563-569．5NikaidoH．Molecularbasisofbacterialoutermem-branepermeabilityrevisitedJ．MicrobiolMolBiolRev2003674593-656．6FourelDHikitaCBollaJMetal．CharacterizationofompFdomainsinvolvedinEscherichiacoliK-12sensitivitytocolicinsAandNJ．JBacteriol1990172103675-3680．7PagesJMJamesCEWinterhalterM．TheporinandthepermeatingantibioticaselectivediffusionbarrierinGram-negativebacteriaJ．NatRevMicrobiol2008612893-903．8BredinJSimonetVIyerRetal．ColicinsspermineandcephalosporinsacompetitiveinteractionwiththeOmpFeyeletJ．BiochemJ2003376Pt1245-252．9WeissMSAbeleUWeckesserJetal．Molecularar-chitectureandelectrostaticpropertiesofabacterialporinJ．Science199125450381627-1630．10WeissMSSchulzGE．Structureofporinrefinedat1.8resolutionJ．JMolBiol19922272493-509．11CowanSWSchirmerTRummelGetal．CrystalstructuresexplainfunctionalpropertiesoftwoE．co-liporinsJ．Nature19923586389727-733．12PezeshkiSChimerelCBessonovANetal．Un-derstandingionconductanceonamolecularlevelanall-atommodelingofthebacterialporinOmpFJ．BiophysJ20099771898-1906．13SchulzGE．Thestructureofbacterialoutermem-braneproteinsJ．BiochimBiophysActa200215652308-317．14CowanSWGaravitoRMJansoniusJNetal．ThestructureofOmpFporininatetragonalcrystalformJ．Structure19953101041-1050．15KefalaGAhnCKrupaMetal．StructuresoftheOmpFporincrystallizedinthepresenceoffoscholine-12J．ProteinSci20101951117-1125．16DhakshnamoorthyBRaychaudhurySBlachowiczLetal．Cation-selectivepathwayofOmpFporinre-vealedbyanomalousX-raydiffractionJ．JMolBi-ol20093962293-300．17BainbridgeGArmstrongGADoverLGetal．Dis-placementofOmpFloop3isnotrequiredforthemembranetranslocationofcolicinsNandAinvivoJ．FEBSLett19984323117-122．18KoebnikRLocherKPVanGelderP．StructureandfunctionofbacterialoutermembraneproteinsbarrelsinanutshellJ．MolMicrobiol2000372239-253．19JeanteurDLakeyJHPattusF．Thebacterialporin820122superfamilysequencealignmentandstructurepre-dictionJ．MolMicrobiol1991592153-2164．20PhalePSPhilippsenAWidmerCetal．RoleofchargedresiduesattheOmpFporinchannelconstric-tionprobedbymutagenesisandsimulationJ．Bio-chemistry200140216319-6325．21YamashitaEZhalninaMVZakharovSDetal．CrystalstructuresoftheOmpFporinfunctioninacolicintransloconJ．EMBOJ200827152171-2180．22BollaJMLazdunskiCPagesJM．TheassemblyofthemajoroutermembraneproteinOmpFofEsche-richiacolidependsonlipidsynthesisJ．EMBOJ19887113595-3599．23RiedGHindennachIHenningU．Roleoflipopo-lysaccharideinassemblyofEscherichiacolioutermembraneproteinsOmpAOmpCandOmpFJ．JBacteriol1990172106048-6053．24YuFMizushimaS．RolesoflipopolysaccharideandoutermembraneproteinOmpCofEscherichiacoliK-12inthereceptorfunctionforbacteriophageT4J．JBacteriol19821512718-722．25HaggeSOdeCockHGutsmannTetal．Porefor-mationandfunctionofphosphoporinPhoEofEsch-erichiacoliaredeterminedbythecoresugarmoietyoflipopolysaccharideJ．JBiolChem20022773734247-34253．ProgressesonStructureofOuterMembraneProteinOmpFfromEscherichiaColiZHAOZhi-ping1NIEXin12LIZai-xin1ZHANGZhi1XIEWan-ru1WANGYi-jun11．SchoolofChemicalandPharmacenticalEngineeringSichuanUniversityofScience＆EngineeringZigong643000China2．SchoolofBiotechnologyEngineeringofChongqingUniversityChongqing400044ChinaAbstractOmp