Bio-rad电击转化仪使用手册(英文版)

GenePulserXcell™ElectroporationSystemInstructionManualCatalogNumbers165-2660,165-2661,165-2662,165-2666,165-2667,and165-2668FortechnicalservicecallyourlocalBio-RadofficeorintheU.S.call1-800-4BIORAD(1-800-424-6723)OntheWebatdiscover.bio-rad.comWarrantyTheGenePulserXcellelectroporationsystemiswarrantedagainstdefectsinmaterialsandworkmanshipfor1year.Ifanydefectsoccurintheinstrumentsoraccessoriesduringthiswarrantyperiod,Bio-RadLaboratorieswillrepairorreplacethedefectivepartsatitsdiscretionwithoutcharge.Thefollowingdefects,however,arespecificallyexcluded:1.Defectscausedbyimproperoperation.2.RepairormodificationdonebyanyoneotherthanBio-RadLaboratoriesoranauthorizedagent.3.Damagecausedbysubstitutingalternativeparts.4.UseoffittingsorsparepartssuppliedbyanyoneotherthanBio-RadLaboratories.5.Damagecausedbyaccidentormisuse.6.Damagecausedbydisaster.7.Corrosioncausedbyimpropersolventorsample.Thiswarrantydoesnotapplytothefollowingparts:FusesForanyinquiryorrequestforrepairservice,contactBio-RadLaboratories.InformBio-Radofthemodelandserialnumberofyourinstrument.IMPORTANT:ThisBio-RadinstrumentisdesignedandcertifiedtomeetEN61010*andtheEMCrequirementsofEN61326(forClassA)safetystandards.Certifiedproductsaresafetousewhenoperatedinaccordancewiththeinstructionmanual.Thisinstrumentshouldnotbemodifiedoralteredinanyway.Alterationofthisinstrumentwill:Voidthemanufacturer'swarrantyVoidtheEN61010safetycertificationCreateapotentialsafetyhazardBio-RadLaboratoriesisnotresponsibleforanyinjuryordamagecausedbytheuseofthisinstrumentforpurposesotherthanthoseforwhichitisintended,orbymodificationsoftheinstrumentnotperformedbyBio-RadLaboratoriesoranauthorizedagent.*EN61010isaninternationallyacceptedelectricalsafetystandardforlaboratoryinstruments.Model:GenePulserXcellCatalogNo.:165-xxxxDateofDelivery:SerialNo.:InvoiceNo.:PurchaseOrderNo.:TableofContentsSection1IntroductionandSafetyInformation:TheGenePulserXcellSystem........................................................................................................11.1GeneralSafetyInformation............................................................................11.2ElectricalHazards........................................................................................21.3MechanicalHazards......................................................................................21.4OtherSafetyPrecautions..............................................................................2Section2UnpackingandSystemInstallation........................................................32.1UnpackingtheSystemComponents............................................................32.2SettinguptheSystem..................................................................................42.2.1SettinguptheGenePulserXcellMainUnitandConnectingtheShockPod(Cat.#s165-2660,165-2661,165-2662,165-2666)........................................................................................42.2.2ConnectingthePCModuletotheGenePulserXcellMainUnit(Cat.#s165-2660,165-2662,and165-2668)..........................52.2.3ConnectingtheCEModuletotheGenePulserXcellMainUnit(Cat.#s165-2660,165-2661,and165-2667)..........................62.2.4ShockPod(Cat.#s165-2660,165-2661,165-2662,and165-2669)........................................................................................6Section3GenePulserXcellOperatingInstructions..............................................83.1SectionOverview..........................................................................................83.2FrontPanelandHomeScreen......................................................................93.2.1DescriptionofKeypad......................................................................93.2.2HomeScreen................................................................................103.2.3HelpScreens................................................................................113.3ManualOperation........................................................................................123.3.1ManualOperation(GuideGuide)....................................................123.3.2ElectroporationusingExponentialDecayPulses............................123.3.3ElectroporationSpecifyingTimeConstant......................................143.3.4ElectroporationusingSquareWavePulses....................................153.3.5ResultsScreens............................................................................173.3.6SavingaProgramfromManualOperation......................................193.3.6ASavinginaLocationwithoutaNamedUserEntry................203.3.6BSavinginaLocationwithaNamedUserEntry....................203.4Pre-SetProtocols........................................................................................213.4.1UsingaPre-setProtocol(QuickGuide)..........................................213.4.2ElectroporationusingaPre-SetProtocol........................................223.4.3ModifyingPre-SetProtocolParameters........................................253.4.4SavingCh