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38620176ENVIRONMENTALSCIENCEVol.38No.6Jun.20171211*12131.1000972.1500403.100097movingbedbiofilmreactorMBBR6、.MBBR45.97g·m-28hNH+4-N86.76%61.96%~78.76%NO-2-N16hNO-2-N0.5mg·L-1.Illumina、MBBRMBBR.NitrospiraNakamurellaSaprospiraceaeComamonadaceaeMBBR.RhabditidanorankMBBRChlorophyceaenorank..X703.1A0250-3301201706-2444-09DOI10.13227/j.hjkx.2016101422016-10-212017-01-16QNJJ201417KJCX201404201991~E-mail453012151@qq.com*E-mailqqray@hotmail.comAmmoniaRemovalRateandMicrobialCommunityStructuresinDifferentBiofiltersforTreatingAquacultureWastewaterZHOUHong-yu12HANMei-lin1QIUTian-lei1*GAOMin1SUNXing-bin2WANGXu-ming131.BeijingAgro-BiotechnologyResearchCenterBeijingAcademyofAgricultureandForestrySciencesBeijing100097China2.CollegeofForestryScienceNortheastForestryUniversityHarbin150040China3.KeyLaboratoryofUrbanAgricultureNorthMinistryofAgricultureBeijing100097ChinaAbstractThreeMBBRsandthreecurtaintypetricklingfiltersCTFswithdifferentcarrierswereoperatedinlab-scalesimulatedRASs.Thecharacteristicsofbiofilmsammoniaremovalratesandmicrobialcommunitiesinsixreactorswerecomparedwitheachother.ComparedwiththebiofilmsofMBBRsthebiofilmsofCTFswereheavierandgrewfaster.TheweightofbiofilmsonCTFswithcarbonfibercarrierswasthemaximum45.97g·m-2andtheammonianitrogenremovalefficiency86.76%ofthisCTFswashigherthantheotherfiltersorreactors61.96%~78.76%.InadditiontheconcentrationoftheaccumulatednitriteinthecarbonfiberCTFswasthelowestinallthesixreactors.Themicrobialcommunitiesofbiofilmsinthesixbiofilterswereevaluatedbythehigh-throughputIllumina-MiSeqsequencingtechnology.TheresultsshowedthatthemicrobialbacteriaandeukaryotecommunityinbiofilmsofCTFswasdifferentfromthatinbiofilmsofMBBRs.Atbothbacteriaandmicro-eukaryotelevelthespeciesrichnessandbiologicaldiversityofbiofilmsinthetricklingfilterswerehigherthanthoseintheMBBRs.OnthecontrarytheSimpsonindexofbacterialcommunityinbiofilmsofMBBRswashigherthanthatinthetricklingfilters.InallthesixbiofiltersNitrospiraandNakamurellawerethedominatedbacterialgenera.SaprospiraceaewasmoreabundantinCTFsthaninMBBRsbutComamonadaceaewasenrichedintheMBBRs.Atthemicro-eukaryotegenuslevelRhabditidanorankgenuswasmoreabundantinCTFswhileChlorophyceaenorankgenuswasmoreabundantintheMBBRs.TheresultsprovideusefulinformationaboutmicrobialecologythatcanbeusedfortheapplicationofCTFsinRAS.KeywordsrecirculatingaquaculturesystemMBBRtricklingfilterammoniaremovalrateIllumina-MiSeqsequencingtechnology3011.、、、、recirculatingaquaculturesystemsRAS2.RAS20803、、、、6、4RAS5.NH+4-NNO-2-N6NH+4-NNO-2-N7.8~10..movingbedbiofilmreactorMBBR6NH+4-NNO-3-N.11.13M1、M2、M3MBBR3F1、F2、F31、2.P1K1K2..1Table1Basicparametersofthefillers/mm/mm/m2·m-3/g·cm-3M1K215106000.96~0.97M2P12545000.95M3K11075000.96~0.971Fig.1Schematicdiagramofexperimentaldevice2Table2Basicparametersofthefiltercloth/m2·m-3/g·m-2F11300~1500220F2900~1100225F3700~9001601.2MBBR1aMBBR13.7cm3L2L30%.1b3L20cm10cm15cm4.6L.1.3C6H12O633.3mg·L-1NH4Cl68.76mg·L-1NH+4-N18mg·L-1NaHCO371.4mg·L-1Na2HPO454423818.85mg·L-11mg·L-1.MnSO4·H2O33.8mg·L-1H3BO349.4mg·L-1ZnSO4·7H2O43.1mg·L-1FeSO4·7H2O97.3mg·L-1CuSO4·5H2O25.0mg·L-1.6mL1‰6LpH7.5~8.00.5L·min-1DO8~8.5mg·L-12d2.pH7.5~8.00.5L·min-14minDO8~8.5mg·L-125℃40h3.8hNH+4-N、NO-2-NNO-3-N.1.4NH+4-N11.NO-2-N11GB/T5750.5-2006.NO-3-N11.DOLDO.pHWTWinoLabpH7310pH.1.51.5.1DNAPCR60dPowerSoilDNAMOBIOLaboraterieInc.CarlsbadCAUSADNA.PCR16SRNAV3DNAIlluminaMiSeq.515F5'-barcode-GTGCCAGCMGCCGCGG-3'907R5'-CCGTCAATTCMTTTRAGTTT-3'8bpbarcode.1295℃2min95℃30s55℃30s72℃30s2572℃5min.20μL4μL5×FastPfuBuffer2μL2.5mmol·L-1dNTPs5μmol·L-10.8μL0.4μLFastPfuPolymerase10ngDNA.3.PCR18sRNAV43NDF5'-GGCAAGTCTGGTGCCAG-3'V4-euk-R25'-ACGGTATCTRATCRTCTTCG-3'131495℃2min3095℃30s55℃30s72℃30s72℃5min.20μL0.2μmol·L-11×PCRBuffer2.5UPfuDNAPolymerase10ngDNA1415.1.5.2IlluminaMiSeq2%AxyPrepDNAGelExtractionKitAxygenBiosciencesUnionCityCAU.S.QuantiFluorTM-STPromegaU.S..IlluminaMiSeq2×250bp2×300bp.1.5.3QIIMEversion1.17UPARSEversion7.1http//drive5.com/uparse/operationalunitsOTUs97%.silvaSSU11516SRNARDPClassifierhttp//rdp.cme.msu.edu/70%16.R3.1.0pheatmap2010.Rprcomp.22.133MBBR10d40dM1、M2、M33M2M1M33M111.9g·L-1M26g·L-1M39.1g·L-1M1M1.F1、F2、F345.97g·m-241.24g·m-26.34g·m-2Zhang17.MBBR64426NH+4-NNO-2-N.3Table3Dryweightsofbiofilmsoncarriers/g/gM1K223.320.811.9g·L-1M2P123.722.56g·L-1M3K129.627.89.1g·L-1F136.1528.97845.97g·m-2F243.937.2641.24g·m-2F317.0716.16.34g·m-22.2NH+4-N2NH+4-N18mg·L-1NH+4-N16h6NH+4-N0.8hNH+4-NNH+4-N86.76%61.96%~78.76%.8hNH+4-N18NH+4-N.NO-2-NNO-2-N.NO-2-NNO-2-N4.18mg·L-1NO-2-N216h0.5mg·L-1.NH+4-NNH+4-NNO-2-N19NH+4-NNO-2-N.NO-3-N6NO-3-NNO-2-NNO-2-NNO-3-N.MBBR2NH+4-N、NO-2-N、NO-3-NFig.2NH+4-NNO-2-NNO-3-Nconcentrationchangesinthebatchexperiments..5、NH+4-NNO-2-N..2.3α20.DNA、656bp2099897%1OTUαcoverage99%.F1~F3Chao1、ACEShannonMBBRM1~M345Simpson21.F1MBBRF2744238F3.2223.4Table4ComparisonofphylotypecoverageanddiversityestimatorsinthebacterialcommunitiesOTUACEChao1ShannonSimpsonF1209986566927100.995.420.009F2209986036516570.995.000.0185F3209985776156180.994.770.0267M1209984234794960.994.440.0267M2209984344855040.994.130.0478M32099839
本文标题:不同生物过滤系统铵态氮转化速率及生物膜特性分析周洪玉
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