好氧锥形流化床生物膜反应器启动与生物膜形成特征研究

1,2,1,2*,BIXiao-tao2,3(11,130012;21,V6T1Z3;31,130021):(A-TFBBR),(A-CFBBR).,,(11d,16d),100~300d.,A-TFBBRA-CFBBR(32?1)Lm(24?2)Lm,.2COD,A-TFBBRCOD95%,A-CFBBRCOD15%.:;;;;:X70311:A:0250-3301(2009)03-0827-07:2008-04-09;:2008-07-21:NaturalSciencesandEngineeringResearchCouncilofCanada(NSERC);:(1980~),,,,E-mail:[e-mail]*,E-mail:[e-mail]-tupandBiofilmFormationinanAerobicConicalFluidized-bedBiofilmReactorDONGShuang-shi1,2,ZHOUDan-dan1,2,BIXiao-tao2,GAOXin3(1.CollegeofEnvironmentandResources,JilinUniversity,Changchun130012,China;2.DepartmentofChemicalandBiologicalEngineering,UniversityofBritishColumbia,Vancouver,V6T1Z3,Canada;3.ChinaNortheastMunicipalEngineeringDesignandResearchInstitute,Changchun130021,China)Abstract:Bothaerobictapered(A-TFBBR)andcylindricalfluidized-bedbiofilmreactors(A-CFBBR)weresetup.Thestar-tupcharacteristics,performance,characteristicsandmicrostructureofthebiofilmwerealsoinvestigatedinbothreactorswithsimilarconditionofstar-tup.Comparedwiththetraditionalmethodthatusingactivatedsludgeasbacteriasource(mostly100-300dforstar-tup),cultivatingbacterianaturallycouldsavetheacclimationtime.Ittookmuchshortertime(11dforthecylindricalbed,while16dforthetaperedbed)tostartupthereactors.Furthermore,thebiofilmthicknessinA-TFBBRandA-CFBBRwasonly(32?1)Lmand(24?2)Lm,respectively.Thebiofilmwasthinnerthanmostofreportedbifilmobtainedbyotherresearchers.BothA-TFBBRandA-CFBBRshowedhighCODremovalcapacity,andCODremovalrateofA-TFBBRwasmorethan95%,15%higherthanthatofA-CFBBR.Keywords:conicalfluidized-bed;star-tup;biofilmformation;biofilmthickness;bacteriaamount2060,,[1~3].(TFBBR).TFBBR[4~6],TFBBR.Wu[7,9,10]Huang[8]0b()215b5b10b.COD,(AN-TFBBR)(AN-CFBBR).AN-TFBBRAN-CFBBR.Parthiban[11]AN-TFBBR,.,(TFBBR),(CFBBR).,TFBBR.30320093ENVIRONMENTALSCIENCEVol.30,No.3Mar.,2009转载中国科技论文在线,.(A-TFBBR).,,.,,(A-TFBBR)(A-CFBBR).11113L(1).,.2.,,.Kureha,016mm,016gPmL,1132gPmL,1100~1300m2Pg.1Fig.1Bacteriasolutioncultivatedequipment112,1.,:5gPL,215gPL015gPL.113,2L1.30e,8mgPL.,.,.,.,,,.24h10%,.,(ABS).,2.,24h.,2LPh1.2.3.4.5.6.7.8.9.10.DO11.pH12.13.2Fig.2Biologicalconical(orcvlindrical)fluidizedbedse-tup(1).45min,COD(220?5)mgPL.8mgPL,37e.,pH815.pH7,,pH615.82830中国科技论文在线(NH4)2SO48.07ZnSO4#7H2O0.70NH4Cl75.17CuSO4#7H2O0.015CaCl2#2H2O8.95CoCl2#6H2O0.15MgCl2#6H2O12.43(NH4)6Mo7O24#4H2O0.0110.055Na2B4O7#10H2O0.066400114.,()-().,.,[7].,[12].))).)))COD.115(DO)pHVWRModelSP80PD..30~50,(1)(dv):dv=3AB2(1),A,B.,...(COD):0122Lm,2mLHACHCOD,,COD2h,HACHDRP2000COD.SEM:3%()1%,P,.,3nm.HitachiS4700.2211.,,24h.24h,,.3.3Fig.3ABSofbacteriasolutionchangedwithculturetime,.,480nm(ABS)01002.ABS,24h01056,72h01214,..72h,..24h,.,.,,,.8293:中国科技论文在线(A-TFBBR)(A-CFBBR),0.14%,A-TFBBR16d,(32?1)Lm.A-CFBBR20%,A-TFBBR(11d),(24?2)Lm.,.,,.4Fig.4Averagethicknessofbiofilmchangedwithstar-tuptime21212COD.5,12d,10d,2COD.COD95%,COD80%.45min,COD220mgPL,2,.,COD15%.5CODFig.5CODremovalratiochangedwithstar-tuptime213(SEM)6,(SEM).,,.SEM,.,(6B).,.6C.,,,.6DSEM..,,.3311,11d,16d,[13,14](100~300d).:¹,83030中国科技论文在线º,,;»,.6Fig.6SEMimagesofbiofilmafterbioreactorstar-tup,,,,..,.,.SEM,211..,SEM,.312(A-TFBBR)COD(A-CFBBR)15%.A-TFBBR.,,.Webster[15,16],.,.,Wu[7,9]Huang[8],A-TFBBRA-CFBBR..313,.,,8313:中国科技论文在线[17~20].,.,Brett[21],100Lm,10Lm..2,[12,22~25].2Table2ReportsonbiofilmthicknessPLm10~100[21]85[25]60~170[12]200[22]150~400[23]48~90[24]7Fig.7SEMimageofactivatedcarbonsurface,()(25~31Lm)..,,,.(7)..,..,;.(),..Seker[12],,.4(1).,.16d11d.(2)COD95%80%..(3)25~31Lm,.:[1]LeveyJrRP,delaGarzaA,JacobsSC,etal.FluidbedconversionofUO3toUF4[J].ChemEngProg,1960,56(3):43-48.[2]DigheSV,BlinnMB,BuggyJJ,etal.Westinghousefluidizedbedcoalgasificationprocessforphosphoricacidfuelcellpowerplant[A].In:ProceedingsoftheIntersocietyEnergyConversionEngineeringConference[C].1981.2:1059-1067.[3]IshiiT.Mult-iparticlecrystalgrowthratesinverticalcones[J].ChemEngSci,1973,28(5):1121-1128.[4]ScottCD,HancherCW.Useofataperedfluidizedbedascontinuousbioreactor[J].BiotechBioeng,1976,18(10):1393-1403.[5]BoeningPH,LarsenVF.Anaerobicfluidizedbedwheytreatment[J].BiotechBioeng,1982,24(11):2539-2556.[6]DenacM,DunnIJ.Packed-andfluidized-bedbiofilmreactorperformanceforanaerobicwastewatertreatment[J].BiotechBioeng,1988,32(2):159-1731[7]WuCS,HuangJS.Bioparticlecharacteristicsoftaperedanaerobicfluidized-bedbioreactors[J].WatRes,1996,30(1):233-241.[8]HuangJS,YanJL,WuCS.Comparativebioparticleandhydrodynamiccharacteristicsofconventionalandtaperedanaerobicfluidized-bedbioreactors[J].JChemTechnolBiotechnol,2000,75(4):269-278.[9]WuCS,HuangJS.ExpansionCharacteristicsofTaperedFluidized-BedBioreactors.AdvancesinBioprocessEngineering[M].83230中国科技论文在线[10]WuCS,HuangJS.Performanceenhancementwithtaperedanaerobicfluidized-bedbioreactors[J].JChemTechBiotechnol,19